姜黄根茎与葡萄叶组合抗金黄色葡萄球菌活性研究

IF 0.2 Q4 PHARMACOLOGY & PHARMACY
Achmad Januar Er Putra, Sutji Kuswarini, Nurmawati Fatimah, Wening Hapsari
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摘要

传染病一直是一个问题,特别是在第三世界国家。由于抗生素处方不受控制,一些细菌对某些抗菌剂产生了耐药性。其中之一是金黄色葡萄球菌。在印度尼西亚,当地有一种智慧,即使用天然产品作为有效的抗菌剂。在过去的几个世纪里,当地人一直使用姜黄和葡萄,似乎是对抗传染病的有效药物。因此,我们想要评价姜黄和葡萄叶联合提取物对金黄色葡萄球菌的活性。材料和方法:采用试管稀释法进行体外试验。试管由7根管子组成;对照管2根,实验管5根。每种提取物的最高用量为1000mg/ml(即姜黄和葡萄分别为1000mg/ml)。每个处理进行5次重复。最低抑菌浓度MIC (Minimum Inhibitory Concentration)和最低杀菌浓度MBC (Minimum Bactericidal Concentration)分别在琼脂平板上划线,370C孵育24小时。结果:复方浸膏颜色混浊,不能测定MIC。除阴性对照外,细菌在5次复制的所有浓度下都能生长,因此无法获得MBC。结论:姜黄与葡萄叶联合提取物对金黄色葡萄球菌无明显抑制作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Activities of Curcuma heyneana Rhizome and Graptophyllum pictum Leaves Combination against Staphylococcus aureus
Introduction: Infectious diseases have always been a problem, especially in the third-world countries. Due to the uncontrolled prescription of antibiotics, several bacteria developed resistance to some antimicrobial agents. One of them is Staphylococcus aureus. There is local wisdom in Indonesia to use natural products as potent antimicrobial agents. Curcuma heyneana and Graptophyllum pictum had been used by the locals in past centuries and seemed to be an effective agent for combatting infectious diseases. Therefore, we want to evaluate the activity of the combined extract of Curcuma heyneana and Graptophyllum pictum against Staphylococcus aureus.. Material and Methods: An in-vitro test by using the test tube dilution. The test tubes consisted of seven tubes; 2 control tubes and 5 experimental tubes. We used 1000mg/ml of each extract component at the highest (i.e., 1000mg/ml for Curcuma heyneana and Graptophyllum pictum, respectively). Five-times replication was conducted for each treatment. MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) were investigated after being streaked at the agar plate and incubated at 370C for 24 hours. Results: We could not determine the MIC because the colour of the combination of the extract was cloudy. The bacteria grew at all concentrations from five-times replication, except for the negative control, therefore MBC could not be obtained. Conclusion: Staphylococcus aureus was not inhibited effectively by combined extract of Curcuma heyneana and Graptophyllum pictum.
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