利用分子标记对土耳其和欧洲产不均等文氏菌分离株遗传多样性的评价

4区农林科学

Tarim Bilimleri Dergisi-journal of Agricultural Sciences Pub Date : 2017-03-14 DOI:10.15832/TBD.10560

S. Kaymak, N. Boyraz, H. Pınar

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引用次数: 1

摘要

苹果痂病(Venturia inaequalis, Cke)是世界上乃至土耳其苹果树的主要病害。这种疾病会对所有土耳其苹果园造成严重的产量损失。利用分子标记技术对土耳其和欧洲苹果痂病(Venturia inaequalis, Cke)分离株的遗传多样性和亲缘性进行了研究。采用RAPD、ISSR、SSR和SRAP标记进行分子分析。标记系统分析结果表明，分离株之间存在较大的分离性和差异。分离株之间的遗传亲缘关系非常接近，由于宿主特异性和地理位置的原因，群体之间的差异不显著。与ISSR和RAPD标记相比，SSR和SRAP标记所组成的群体数量更多。使用SSR和SRAP标记可提供更多信息，因为它们易于使用、可重复性和特异性。

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The Assessment of Genetic Diversity of Venturia inaequalis Isolates Obtained from Turkey and Europe by Molecular Markers

Apple scab (Venturia inaequalis (Cke) Wint) is the main disease of apple trees not only in the world but also in Turkey. The disease can cause severe yield losses in all Turkish apple orchards. Genetic diversity and relatedness of apple scab (Venturia inaequalis (Cke) Wint) isolates collected from Turkey and Europe were investigated in this study through molecular markers. RAPD, ISSR, SSR and SRAP markers were used in the molecular studies. Results obtained by marker system revealed that isolates were quite separated from each other and it is appeared to exist a variation between them. Genetic relatedness between the isolates are very close to each other, and difference among the groups is not significant due to host specificity and geographical location. The groups are consisted of more number of isolates when SSR and SRAP markers are used compared with ISSR and RAPD markers. Using SSR and SRAP markers are preferable to provide more informative outcomes because of ease of use, repeatability and specificity.

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来源期刊

Tarim Bilimleri Dergisi-journal of Agricultural Sciences 农林科学-农业综合

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Journal of Agricultural Sciences (JAS) is an international, double-blind peer-reviewed, open-access journal, published by the Faculty of Agriculture, Ankara University. The journal invites original research papers containing new insight into any aspect of Agricultural Sciences that are not published or not being considered for publication elsewhere. Preliminary, confirmatory or inconclusive research, review articles, case and local studies and works presenting taxonomy will not be published.