冷藏时间对孟加拉沿海地区本地雄鹿精液精子质量的影响

M. Swarna, S. Biswas, D. Biswas, N. G. Saha, A. Paul
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引用次数: 0

摘要

背景:精液特征评估是精液保存前的重要步骤。本研究旨在收集、评估和保存孟加拉国沿海地区的土著雄鹿精液。方法:研究于2021年7月至2022年6月在帕图阿卡利科技大学医学、外科和产科医学与动物生殖生物技术实验室进行。我们根据不回头率,怀孕率,以及包括年龄,体重,阴囊周长,睾丸附睾长度在内的各种参数选择了四美元。这些选定的动物接受了维生素、矿物质、驱虫药和浓缩饲料的治疗。采用人工阴道法采集精液,采集后记录并分析精液的颜色、气味、体积、粘度、质量活性、稠度、浓度和单个精子活力等数据。结果:雄鹿精液呈乳白色,有鱼腥味。4雄犬阴囊围(SC)均值(±SD)为20.43±0.52,睾丸附睾长度(TEL)均值(11.03±0.59)。buck- 2 (B-2)和buck-1 (B-1)的体积显著高于buck-3 (B-3)和buck (B-4) (p<0.05)。B-1的质量活性显著高于B-2、B-3和B-4 (p<0.05)。B-2和B-1的一致性显著高于B-3和B-4 (p<0.05)。与B-3和B-4相比,B-2和B-1的浓度最高。在个体精子活力方面,B-1(81.0±05.16)显著高于B-2(71.0±03.94)、B-3(66.0±03.94)和B-4(80.0±07.45)(p<0.05)。关于冷冻时间,在4°C的储存期间观察到精子活力显著降低。这表明精子活力随冷却时间的变化而显著变化(p≤0.000)。结论:本研究提示,在4°C条件下,个体进行性精子活力逐渐降低,但仍适合人工授精72 h(≥50±7.65)。B-1和B-2在精液评估和保存方面表现出相对有希望的结果。冷冻精液授精后雌牛的受孕率有待进一步研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Impact of chilling duration on sperm quality of indigenous buck semen in the Coastal Area of Bangladesh
Background: Assessing semen characteristics is an important step before semen preservation. This study aimed to collect, evaluate, and preserve indigenous buck semen in the coastal area of Bangladesh. Methods: The study was conducted at the Theriogenology and Animal Reproductive Biotechnology Laboratory, Department of Medicine, Surgery and Obstetrics, Patuakhali Science and Technology University, from July 2021 to June 2022. We selected four bucks based on non-return rate, pregnancy rate of does, and various parameters including age, body weight, scrotal circumference, and testicular epididymal length. These selected animals received a regimen of vitamins, minerals, anthelmintics, and concentrated feed. Semen was collected using the artificial vagina method, and data on color, odor, volume, viscosity, mass activity, consistency, concentration, and individual sperm motility were recorded and analyzed after collection. Results: The semen of bucks exhibited a creamy white color and a fishy odor. The mean (±SD) of scrotal circumference (SC) of four bucks was 20.43±0.52, while the testicular epididymal length (TEL) was 11.03±0.59. Buck-2 (B-2) and buck-1 (B-1) showed significantly (p<0.05) higher volumes than buck-3 (B-3), and buck (B-4). B-1 also displayed significantly (p<0.05) higher mass activity compared to B-2, B-3, and B-4. In addition, B-2 and B-1 showed significantly (p<0.05) greater consistency than B-3, and B-4. The highest concentrations were observed in B-2 and B-1 compared to B-3 and B-4. In terms of individual sperm motility, B-1 showed significantly (p<0.05) higher motility (81.0±05.16) than B-2 (71.0±03.94), B-3 (66.0±03.94), and B-4 (80.0±07.45). Regarding chilling duration, a significant reduction in sperm motility was observed during storage at 4°C. This indicated that sperm motility varied significantly (p ≤ 0.000) with varying chilling durations. Conclusions: The study suggests that the individual progressive sperm motility at 4°C gradually decreases but remains suitable for artificial insemination up to 72 h (≥50±7.65). B-1 and B-2 demonstrated relatively promising results in semen evaluation and preservation. Further study is necessary to determine the pregnancy rate of does after insemination with chilled semen.
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