A.J. Bell , M. Wrightham , F.K. Stevenson , T.J. Hamblin
{"title":"淋巴因子激活的杀伤细胞在一个完全封闭的系统中产生","authors":"A.J. Bell , M. Wrightham , F.K. Stevenson , T.J. Hamblin","doi":"10.1016/S0278-6222(87)80047-5","DOIUrl":null,"url":null,"abstract":"<div><p>We have assessed a totally closed system for the generation of lymphokine activated killer (LAK) cells in a district general hospital. The elements of the system are (1) collection of mononuclear cells using the Haemonetics V50 either by lymphocytapheresis and Ficoll-Hypaque separation or by the lymphocyte surge protocol, (2) culture of LAK cells in 1 liter plastic bags, and (3) connections between plastic tubes made with the sterile connecting device.</p><p>The lymphocyte surger protocol produces an acceptable lymphocyte concentrate more quickly than the standard lymphocytapheresis method. It was possible to generate LAK cells from patients with lymphoma even when their blood was heavily contaminated by circulating tumor cells.</p><p>Ways of using LAK cells in conjunction with monoclonal antibodies are discussed.</p></div>","PeriodicalId":101030,"journal":{"name":"Plasma Therapy and Transfusion Technology","volume":"8 4","pages":"Pages 371-376"},"PeriodicalIF":0.0000,"publicationDate":"1987-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0278-6222(87)80047-5","citationCount":"4","resultStr":"{\"title\":\"Generation of lymphokine activated killer cells in a totally closed system\",\"authors\":\"A.J. Bell , M. Wrightham , F.K. Stevenson , T.J. Hamblin\",\"doi\":\"10.1016/S0278-6222(87)80047-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>We have assessed a totally closed system for the generation of lymphokine activated killer (LAK) cells in a district general hospital. The elements of the system are (1) collection of mononuclear cells using the Haemonetics V50 either by lymphocytapheresis and Ficoll-Hypaque separation or by the lymphocyte surge protocol, (2) culture of LAK cells in 1 liter plastic bags, and (3) connections between plastic tubes made with the sterile connecting device.</p><p>The lymphocyte surger protocol produces an acceptable lymphocyte concentrate more quickly than the standard lymphocytapheresis method. It was possible to generate LAK cells from patients with lymphoma even when their blood was heavily contaminated by circulating tumor cells.</p><p>Ways of using LAK cells in conjunction with monoclonal antibodies are discussed.</p></div>\",\"PeriodicalId\":101030,\"journal\":{\"name\":\"Plasma Therapy and Transfusion Technology\",\"volume\":\"8 4\",\"pages\":\"Pages 371-376\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1987-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0278-6222(87)80047-5\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plasma Therapy and Transfusion Technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0278622287800475\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plasma Therapy and Transfusion Technology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0278622287800475","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Generation of lymphokine activated killer cells in a totally closed system
We have assessed a totally closed system for the generation of lymphokine activated killer (LAK) cells in a district general hospital. The elements of the system are (1) collection of mononuclear cells using the Haemonetics V50 either by lymphocytapheresis and Ficoll-Hypaque separation or by the lymphocyte surge protocol, (2) culture of LAK cells in 1 liter plastic bags, and (3) connections between plastic tubes made with the sterile connecting device.
The lymphocyte surger protocol produces an acceptable lymphocyte concentrate more quickly than the standard lymphocytapheresis method. It was possible to generate LAK cells from patients with lymphoma even when their blood was heavily contaminated by circulating tumor cells.
Ways of using LAK cells in conjunction with monoclonal antibodies are discussed.
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