过量的叶酸摄入与妊娠期营养不良相结合会改变后代的行为和大脑单胺谱

IF 1.3 4区 医学 Q3 PEDIATRICS
Tetsuo Ono, Kodai Hino, Tomoko Kimura, Yasuhiro Uchimura, Takashi Ashihara, Takako Higa, Hideto Kojima, Takashi Murakami, Jun Udagawa
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引用次数: 1

摘要

妊娠期饮食中增加叶酸可降低后代神经发育障碍风险;然而,目前尚不清楚母亲过量摄入叶酸是否会损害后代的大脑功能。我们研究了在整个妊娠期过量摄入叶酸是否会改变妊娠早期营养不良的雄性后代的行为(E5.5-E11.5)。将白羊分为4组:对照组(CON, 2 mg叶酸/kg食物)、过量叶酸强化组(FF, 10 mg叶酸/kg食物)、营养不足组(UN,从e5.5 ~ e11.5减少40%食物)、过量叶酸强化加营养不足组(UN-FF)。在9周龄时,过量的母体叶酸强化诱导了开阔场地的多动症和高加迷宫中焦虑样行为的降低。这些行为变化伴随着前额皮质(PFC)、杏仁核中去甲肾上腺素的减少,以及中脑背侧(DM)、PFC和杏仁核中5-羟色胺(5-HT)的减少,其中5-HT神经元从DM投射出来。此外,典型判别分析包括PFC中的多巴胺和DOPAC浓度,PFC、杏仁核和脑轴中的去甲肾上腺素浓度,以及杏仁核和DM中的5-HT和5-HIAA浓度,CON、FF、UN和UN-FF组的后代正确分类率为73.5%。第一个判别函数主要根据营养状况进行分类,第二个判别函数主要根据叶酸摄入量进行分类。我们的研究表明,母亲营养不良和过量叶酸摄入导致的大脑单胺谱的联合转变与后代的行为改变有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Excessive folic acid intake combined with undernutrition during gestation alters offspring behavior and brain monoamine profiles

Dietary folic acid augmentation during gestation reduces neurodevelopmental disorder risk in offspring; however, it is still unclear if excessive maternal folic acid intake can impair brain function in offspring. We examined if excessive folic acid intake throughout gestation altered the behavior of male offspring under poor nutrition during early gestation (E5.5–E11.5). Dams were divided into four groups: control (CON, 2 mg folic acid/kg of food), excessive folic acid fortification (FF, 10 mg folic acid/kg of food), undernutrition (UN, 40% food reduction from E5.5–E11.5), and excessive folic acid fortification plus undernutrition (UN-FF). Excess maternal folic acid fortification induced hyperactivity in the open-field and lower anxiety-like behavior in the elevated plus maze at 9 weeks of age. These behavioral changes were accompanied by reduced dopamine in the prefrontal cortex (PFC), norepinephrine in the amygdala, and 5-hydroxytryptamine (5-HT) in the dorsal midbrain (DM), PFC, and amygdala where 5-HT neurons project from the DM. Furthermore, canonical discriminant analysis, including dopamine and DOPAC concentrations in the PFC, norepinephrine concentrations in the PFC, amygdala, and pons, and 5-HT and 5-HIAA concentrations in the amygdala and DM, correctly classified 73.5% of the offspring in CON, FF, UN, and UN-FF groups. The first discriminant function mainly classified groups based on nutritional status, whereas the second function mainly classified groups based on folic acid intake. Our study suggests that combined transformations of brain monoamine profiles by maternal undernutrition and excess folic acid intake is involved in the behavioral alteration of offsprings.

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来源期刊
Congenital Anomalies
Congenital Anomalies PEDIATRICS-
自引率
0.00%
发文量
49
审稿时长
>12 weeks
期刊介绍: Congenital Anomalies is the official English language journal of the Japanese Teratology Society, and publishes original articles in laboratory as well as clinical research in all areas of abnormal development and related fields, from all over the world. Although contributions by members of the teratology societies affiliated with The International Federation of Teratology Societies are given priority, contributions from non-members are welcomed.
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