球孢白僵菌对黄粉蚧及其寄生蜂赤眼蜂的防治作用

B. Tuncsoy, F. Bueno-Pallero, L. Dionísio, Luís Neto, Pınar Ozalp
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引用次数: 1

摘要

本实验采用球孢白僵菌(Beauveria bassiana)作为一种昆虫病原真菌,以测定其对黑蜡蚧及其寄生蜂赤眼蜂(Trichogramma cacoeciae)的作用。分别以105、106、107、108个分生孢子/mL注射至末龄大孢子菌。为研究球孢白僵菌对球芽胞杆菌的杀伤作用,将108个分生孢子/mL施用于球芽胞菌卵中。105和106个分生孢子/mL注射组未见死亡,107和108个分生孢子/mL注射组有死亡幼虫。被皮内黑斑的发生率日益增加。球孢白僵菌侵染后,棉铃虫卵的孵化率显著降低。寄生率和羽化率不受球孢白僵菌的影响。通过监测球孢白僵菌的寄生率和羽化率,确定了球孢白僵菌对球虫的生物安全性。结果表明,球孢白僵菌对该卵类寄生物是安全的。但对粗粉蚧幼虫的死亡率和卵的孵化率有显著影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of Beauveria bassiana on Galleria mellonella L. (Lepidoptera:Pyralidae) and its parasitoid Trichogramma cacoeciae
Beauveria bassiana, which is a species of entomopathogenic fungus, used for this experiment in order to determine the effects on Galleria mellonella and its parasitoid Trichogramma cacoeciae. 105, 106, 107 and 108 conidia/mL were applied to the last instar of G. mellonella by injecting the suspensions. To determine the effect of B. bassiana on T. cacoeciae, 108 conidia/mL were applied to the G. mellonella eggs. No mortality was observed in 105 and 106 conidia/mL of B. bassiana injected group, whereas death larvae was determined in 107 and 108 conidia/mL injected groups. Prevalence of black spots within the integument increased day by day. Hatchability of G. mellonella eggs were significantly decreased when B. bassiana applied to the G. mellonella eggs. Parasitism and emergence ratio were unaffected by B. bassiana. The biosafety of B. bassiana against T. cacoeciae was determined by monitoring the parasitization and emergence ratio. It was detected B. bassiana was safe to this egg parasitoid. However, there is a significant effect on mortality of G. mellonella larvae and hatchability of G. mellonella eggs.
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