马鞭草叶片甲醇提取物的体外药理活性:一个生长在孟加拉国达卡的品种

M. Alam, S. Akash
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摘要

被称为牙痛植物,属于菊科。它被当作治疗肺结核、疾病、咳嗽、风湿病和疾病的药物。本文采用体外模型研究了甲醇提取物的抗氧化、细胞毒、抗菌和溶栓活性。利用DPPH和还原功率试验估计其抗氧化活性为trolox当量抗氧化能力。采用盐水虾致死性生物测定法测试了植物提取物的细胞毒性作用,采用凝块破坏法测试了其溶血栓活性,采用圆盘扩散法测试了其对四种不同的革兰氏阳性和革兰氏阴性细菌的抗菌活性。标准抗坏血酸对DPPH的IC50值为7.8µg/ml,叶片甲醇提取物的IC50值为198.34µg/ml,与抗坏血酸相比作用不大。作为阳性对照的链激酶溶栓试验显示,溶栓活性为88.49%,其中提取物的溶栓活性为18.69%。本实验中细胞毒性测定的LC结果样品为1.431µg/mL,可作为活性较低处理。此外,提取物对革兰氏阴性和革兰氏阳性细菌染色均表现出低至中等的抗菌活性(抑制区-10-26 mm)。模拟弧菌(Vibrio Mimicus)染色的活性最高,其生长抑制区直径范围为23。目前的综述表明,叶提取物可能用作抗氧化和溶栓活性的来源,以及抗菌和抗癌物质的重要来源。进一步的研究需要评估叶片的体内药理活性,以确定必要的代谢物和潜在的机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In vitro pharmacological activities of methanol extract of Acmella oleracea leaves: A variety grown in Dhaka, Bangladesh
is known as toothache plant belongs to the family of Asteraceae. It is treated as a medicinal remedy like tuberculosis, illness, cough, rheumatism, and illness. This study was concentrated on the antioxidant, cytotoxic, antimicrobial & thrombolytic activities of methanol extract of leaves using in vitro model. The antioxidant activity was estimated as trolox equivalent antioxidant capacity utilizing the DPPH and reducing power tests. The plant extract was tested for its cytotoxic action using a brine shrimp lethality bioassay, thrombolytic activity using clot disruption, and antibacterial activity using a disc diffusion assay technique against four distinct gram-positive also gram-negative bacteria. ICvalue of standard ascorbic acid for DPPH was 7.8µg/ml and the IC50 of the methanol extract of leaves was 198.34µg/ml that was moderate effect at all compare with ascorbic acid. Thrombolytic assay of Streptokinase as a positive control showed 88.49% where the extract shows 18.69% lytic activity shows the assay. In this study, the sample of LC results of cytotoxicity assay was 1.431 µg/mL which can be treated as less activity. Moreover, the extracts showed low to moderate antibacterial activity against both gram-negative and gram-positive bacterial stains (zone of inhibition-10-26 mm). Vibrio Mimicus bacteria stains exhibited the highest level of activity, with a range of 23 for the diameter of the growth inhibition zone. The current review shows leaf extracts of may be used as a source of antioxidant and thrombolytic activity, as well as a significant source of antibacterial and anticancer substances. Further research is required to evaluate in-vivo the pharmacological activity of leaves in order to identify the essential metabolites and potential mechanisms.
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