臭氧气体和低压对手术针头中金黄色葡萄球菌失活的影响

IF 2.1 4区 环境科学与生态学 Q3 ENGINEERING, ENVIRONMENTAL
Vera Lúcia Taba, H. C. Carvalho, Túlia de Souza Botelho, A. B. Fernandes, Carlos José de Lima, A. Villaverde
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引用次数: 2

摘要

环氧乙烷和伽马辐射是两种公认的手术材料灭菌工艺;然而,它们分别具有毒性和高成本。臭氧气体O3(g)已被有效地用于消毒和灭菌。低压(LP)可以促进水的蒸发,当蒸汽压降至水饱和压力以下时,水饱和压力在20℃(实验温度)为2337 Pa。用于污染手术针头的微生物是金黄色葡萄球菌,这是一种革兰氏阳性细菌,其细胞质主要是水。60份SN样品分为6组,每组10份:阴性对照组(无菌SN)、阳性对照组(实验污染SN)、LP组、LP与O3(g)联合组和O3(g)单独组。CFU/mL计数显示LP+O3(g)和O3(g)组的杀微生物效果。这是一种创新的微生物灭活方案,主要是因为LP的应用。在未来,该技术可用于SN和其他医疗器械的灭菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Inactivation of Staphylococcus aureus in Surgical Needles by Exposure to Ozone Gas and Low Pressure
ABSTRACT Ethylene oxide and gamma irradiation are two well-established sterilizing processes for surgical materials; however, they present toxicity and high cost, respectively. Ozone Gas, O3(g), has been used effectively for disinfection and sterilization. Low pressure (LP) can promote water vaporization whenever the vapor pressure drops below the water saturation pressure, which at 20 °C (experimental temperature) is 2337 Pa. The microorganism used to contaminate the surgical needles (SN) was Staphylococcus aureus, a gram-positive bacterium whose cytoplasm is mostly water. Sixty SN samples were divided into six groups of 10: negative control (sterile SN), positive control (experimentally contaminated SN), LP group, two groups associating LP and O3(g), and O3(g) alone group. The CFU/mL count demonstrated microbicide efficacy of LP+O3(g) and O3(g) groups. This is an innovative protocol for microbial inactivation, mainly because of LP application. In the future, this technique can be evaluated for sterilization of SN and other medical devices.
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来源期刊
Ozone: Science & Engineering
Ozone: Science & Engineering 环境科学-工程:环境
CiteScore
5.90
自引率
11.10%
发文量
40
审稿时长
2 months
期刊介绍: The only journal in the world that focuses on the technologies of ozone and related oxidation technologies, Ozone: Science and Engineering brings you quality original research, review papers, research notes, and case histories in each issue. Get the most up-to date results of basic, applied, and engineered research including: -Ozone generation and contacting- Treatment of drinking water- Analysis of ozone in gases and liquids- Treatment of wastewater and hazardous waste- Advanced oxidation processes- Treatment of emerging contaminants- Agri-Food applications- Process control of ozone systems- New applications for ozone (e.g. laundry applications, semiconductor applications)- Chemical synthesis. All submitted manuscripts are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees.
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