小儿口腔缺氧硒单胞菌的唾液筛查

R. Davidowitz, K. Howard, K. Kingsley
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引用次数: 0

摘要

口腔微生物组可能受到患者药物、疾病状况和全身性疾病的影响。无氧硒单胞菌是一种无氧、可运动、不形成孢子的革兰氏阴性杆状菌,与牙周病和其他疾病(包括肥胖)反复相关。由于缺乏关于口腔患病率的证据,本研究的目的是通过从口腔取样唾液以筛查该病原体来评估耐氧链球菌的患病率。利用现有的唾液库,采用定量实时聚合酶链反应(qPCR)分离和筛选DNA。对研究样本和qPCR结果进行人口统计学分析。大约一半的样本(n=96)来自女性(51%),大多数来自西班牙裔患者(62.5%)。通过DNA分离和qPCR筛选,发现37.5% (n=35)的患者携带缺氧链球菌DNA,其中成人(n=22或22.9%)比儿童(n=13或13.5%)更为普遍。本研究提供了新的信息,关于口腔流行的S. noxia在儿童和成人人群从牙科学校的人口。这些数据是该微生物总体流行病学分析的重要组成部分,并可能提供与这些人群中存在的牙周风险或其他健康问题相关的一些初步信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Salivary screening for Selenomonas noxia in the oral cavity of pediatric patients
The oral microbiome may be affected by patient medications, disease conditions and systemic disorders. Selenomonas noxia is an anaerobic, motile, non spore-forming, gram-negative rod that has been repeatedly associated with periodontal disease and other disorders, including obesity. Based upon the paucity of evidence regarding oral prevalence, the objective of was to evaluate S. noxia prevalence by sampling saliva from the oral cavity to screen for this pathogen. Using an existing saliva repository, DNA was isolated and screened using quantitative real-time polymerase chain reaction (qPCR). Demographic analysis of study samples and qPCR results was also performed. Approximately half of the samples (n=96) were derived from females (51%) and the majority were from Hispanic patients (62.5%). Following DNA isolation and qPCR screening 37.5% (n=35) were found to harbor S. noxia DNA, which was more prevalent among the samples derived from adults (n=22 or 22.9%) than pediatric patients (n=13 or 13.5%). This study provides novel information regarding the oral prevalence of S. noxia among both pediatric and adult populations from a dental school population. These data are an important part of the overall epidemiologic analysis of this organism and may provide some initial information regarding the risk for periodontal or other health issues related to the presence among these populations.
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