马来西亚半岛吉打州兰卡威岛兰氏蛙皮肤分泌物蛋白质组学(无尾目:兰氏蛙科)(Boulenger, 1882

IF 0.5 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Current Proteomics Pub Date : 2022-12-09 DOI:10.2174/1570164620666221209121936

D. Ong, S. Shahrudin, M. Ismail

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引用次数: 0

摘要

蛋白质组学研究的进展为研究带来了各种好处。然而，在马来西亚，蛋白质组学研究仍处于早期阶段。从特穆伦瀑布溪流中采集了4只成年腺斑蛙进行蛋白质组学分析。收集、提取这些青蛙的皮肤分泌物，并使用液相色谱-质谱法分析其蛋白质抗菌肽化合物和生物医学潜力。从其皮肤分泌物中鉴定出46种蛋白质。它们含有未经审查的蛋白质、酶、amp、受体、调节蛋白、转运蛋白、激素和发育蛋白。此外，还鉴定出11种AMP，包括esculentin-2、brevinin 1等AMP。这些蛋白质和amp具有广泛的生物医学重要性，如抗菌剂、抗肿瘤、抗癌、抗病毒、伤口愈合、抗炎症、抗衰老和维持体内平衡。

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Proteomics of the skin secretion of Pulchrana glandulosa (Anura: Ranidae) (Boulenger, 1882), Langkawi Island, Kedah, Peninsular Malaysia

The advancement of proteomics studies leads to various benefits in research. However, in Malaysia, proteomics studies are still in their early stage. Four adult Pulchrana glandulosa individuals were collected from the stream of Temurun Waterfall for their proteomics information. These frogs’ skin secretions were collected, extracted, and analysed for their protein antimicrobial peptide compounds and biomedical potentials using liquid chromatography-mass spectrometry. Forty-six proteins had been identified from the skin secretion of this species. They contained unreviewed proteins, enzymes, AMPs, receptors, regulatory, transport, hormone, and developmental proteins. In addition, 11 AMP had been identified, consisting of esculentin-2, brevinin 1, and other AMPs. These proteins and AMPs have a wide range of biomedical importance, such as antimicrobials, anti-tumour, anti-cancerous, anti-viral, wound healing, anti-inflammation, anti-ageing and maintaining homeostasis.

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来源期刊

Current Proteomics BIOCHEMICAL RESEARCH METHODS-BIOCHEMISTRY & MOLECULAR BIOLOGY

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

>0 weeks

期刊介绍： Research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed in-depth/mini review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry. Current Proteomics publishes in-depth/mini review articles in all aspects of the fast-expanding field of proteomics. All areas of proteomics are covered together with the methodology, software, databases, technological advances and applications of proteomics, including functional proteomics. Diverse technologies covered include but are not limited to: Protein separation and characterization techniques 2-D gel electrophoresis and image analysis Techniques for protein expression profiling including mass spectrometry-based methods and algorithms for correlative database searching Determination of co-translational and post- translational modification of proteins Protein/peptide microarrays Biomolecular interaction analysis Analysis of protein complexes Yeast two-hybrid projects Protein-protein interaction (protein interactome) pathways and cell signaling networks Systems biology Proteome informatics (bioinformatics) Knowledge integration and management tools High-throughput protein structural studies (using mass spectrometry, nuclear magnetic resonance and X-ray crystallography) High-throughput computational methods for protein 3-D structure as well as function determination Robotics, nanotechnology, and microfluidics.