Development of Nano-KIT for Synthesis of Silver and Gold Nanostructures with Emphasis on Biological Applications

Nano-KIT consist of few standard chemicals (solvents)/constitutes that can be added sequentially by given protocol. Spherical silver (30 nm) and gold (25–30 nm) nanostructure were synthesized instantly in (5–10 min). Surface plasmon resonance for silver 413 nm and gold 545 nm were confirmed synthesis. Synthesized silver has stability more than 36 months in cold condition. Dynamic Light Scattering (DLS), X-ray Diffraction (XRD) and High Resolution-Transmission Electron Microscopy (HR-TEM) revealed 30 nm (silver) and 25–30 nm (gold). Nano-KIT can be used for variety of application in Nano research areas like biomedical and industrial. Synthesized silver nanostructure were analysed with its molecular interaction between Bovine Serum Albumin (BSA). Biocompatibility assays with Red Blood Cells (RBC), number of binding sites, thermodynamic parameters i.e., free energy, enthalpy and entropy were studied.