用x射线衍射表征去卵巢大鼠骨质减少模型的骨重塑和纳米复合材料

IF 0.7 Q4 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
N. Kostyshyn, M. Gzhegotskyi, L. Kostyshyn, O. Yarova, Y. Kulyk, S. Mudry
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引用次数: 0

摘要

久坐不动的生活方式和生理性更年期是骨质减少的危险因素,尤其是老年人。然而,在年轻人中也可以观察到骨密度下降,例如,由于手术干预后女性性激素的缺乏,特别是卵巢切除术。我们的研究使我们能够评估全身振动在防止卵巢切除大鼠骨质减少模型中骨矿物质密度损失的功效。因此,对切除卵巢的年轻雌性大鼠(n = 18)采用加速度水平为0.3 g,频率为50 Hz的全身振动。实验持续24周,暴露时间为每天30分钟,每周5次。通过x射线衍射评估胫骨矿物成分的损失。通过测定血液中甲状旁腺激素、降钙素、钙和磷等激素来评估骨重塑。x射线衍射是实验中评价骨组织纳米复合材料结构的一种有效方法。在本文中,我们应用该方法来测定卵巢切除术后骨矿物质量的损失以及这种情况下全身振动的影响。在卵巢切除术组中,从第16周开始,矿物质成分的体积与对照组相比已显著减少(p <0.05)。然而,在卵巢切除术+全身振动组中,与对照组相比,在调查的8-16周期间,矿物质成分的损失不显著。在第24天,光谱与去卵巢大鼠组几乎没有差异。同时,去卵巢大鼠组激素水平发生变化。需要强调的是,上述全身振动参数不会造成严重的骨损伤或进一步的负面后果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Using X-ray diffraction in characterization of bone remodeling and nanocomposites in ovariectomized rats osteopenia model
Abstract Sedentary lifestyle and physiological menopause are among the risk factors of osteopenia, especially in elderly people. However, bone mineral density decrease can also be observed in young individuals, for instance, due to deficiency of female sex hormones after surgical interventions, particularly ovariectomy. Our research enabled us to assess the efficacy of whole-body vibration in preventing the loss of bone mineral density in the ovariectomy rat osteopenia model. Thus, whole-body vibration with acceleration level 0.3 g and frequency 50 Hz was used on young female rats, which had been subjected to ovariectomy (n = 18). It had been conducted for 24 weeks, exposure time – 30 minutes per day, 5 times a week. Assessment of mineral component loss of the tibia was performed by means of X-ray diffraction. Bone remodeling was assessed by determining hormones: parathyroid hormone and calcitonin, Ca and P in the blood. X-ray diffraction is an effective method, which enables the evaluation a nanocomposites structure of the bone tissue in the experiment. In the article, we applied this method to determine the loss of bone mineral mass after ovariectomy and the impact of whole-body vibration under such conditions. In the ovariectomy group, the volume of a mineral component significantly decreased starting already from the 16th week (р<0.05) versus control. However, in the group with ovariectomy + whole-body vibration, the loss of a mineral component was insignificant during 8-16 weeks of the investigation, compared with the control group. On the 24th day, the spectrums almost did not differ from ovariectomized rats group. Meanwhile, hormone levels changed in ovariectomized rats group. It should be emphasized that the aforementioned whole-body vibration parameters do not cause severe bone damage or further negative consequences.
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来源期刊
Polish Journal of Medical Physics and Engineering
Polish Journal of Medical Physics and Engineering RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-
CiteScore
1.30
自引率
0.00%
发文量
19
期刊介绍: Polish Journal of Medical Physics and Engineering (PJMPE) (Online ISSN: 1898-0309; Print ISSN: 1425-4689) is an official publication of the Polish Society of Medical Physics. It is a peer-reviewed, open access scientific journal with no publication fees. The issues are published quarterly online. The Journal publishes original contribution in medical physics and biomedical engineering.
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