鸡和小鼠肢体发育过程中Sim1和Sim2的表达。

P. Coumailleau, D. Duprez
{"title":"鸡和小鼠肢体发育过程中Sim1和Sim2的表达。","authors":"P. Coumailleau, D. Duprez","doi":"10.1387/ijdb.082659pc","DOIUrl":null,"url":null,"abstract":"The Drosophila Single minded (Sim) transcription factor is a master regulator of cell fate during midline development. The homolog mouse Sim1 and Sim2 genes are important for central nervous system development. Loss of mSim1 activity leads to an absence of specific neuroendocrine lineages within the hypothalamus, while overexpression of mSim2 leads to behavioural defects. We now provide evidence that vertebrate Sim genes might be important for limb muscle formation. We have examined by in situ hybridisation the expression of the Sim1 and Sim2 genes during limb development in chick and mouse embryos. The expression of both Sim genes is mainly associated with limb muscle formation. We found that each Sim gene has a similar temporal and spatial expression pattern in chick and mouse embryonic limbs, although with some differences for the Sim2 gene between species. In chick or mouse embryonic limbs, Sim1 and Sim2 display non-overlapping expression domains, suggesting an involvement for Sim1 and Sim2 proteins at different steps of limb muscle formation. Sim1 gene expression is associated with the early step of muscle progenitor cell migration in chick and mouse, while the Sim2 gene is expressed just after the migration process. In addition, chick and mouse Sim2 gene expression is enhanced in limb ventral muscle masses versus dorsal ventral muscle masses. Our results provide a basis for further functional analysis of the Sim genes in limb muscle formation.","PeriodicalId":94228,"journal":{"name":"The International journal of developmental biology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"34","resultStr":"{\"title\":\"Sim1 and Sim2 expression during chick and mouse limb development.\",\"authors\":\"P. Coumailleau, D. Duprez\",\"doi\":\"10.1387/ijdb.082659pc\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The Drosophila Single minded (Sim) transcription factor is a master regulator of cell fate during midline development. The homolog mouse Sim1 and Sim2 genes are important for central nervous system development. Loss of mSim1 activity leads to an absence of specific neuroendocrine lineages within the hypothalamus, while overexpression of mSim2 leads to behavioural defects. We now provide evidence that vertebrate Sim genes might be important for limb muscle formation. We have examined by in situ hybridisation the expression of the Sim1 and Sim2 genes during limb development in chick and mouse embryos. The expression of both Sim genes is mainly associated with limb muscle formation. We found that each Sim gene has a similar temporal and spatial expression pattern in chick and mouse embryonic limbs, although with some differences for the Sim2 gene between species. In chick or mouse embryonic limbs, Sim1 and Sim2 display non-overlapping expression domains, suggesting an involvement for Sim1 and Sim2 proteins at different steps of limb muscle formation. Sim1 gene expression is associated with the early step of muscle progenitor cell migration in chick and mouse, while the Sim2 gene is expressed just after the migration process. In addition, chick and mouse Sim2 gene expression is enhanced in limb ventral muscle masses versus dorsal ventral muscle masses. Our results provide a basis for further functional analysis of the Sim genes in limb muscle formation.\",\"PeriodicalId\":94228,\"journal\":{\"name\":\"The International journal of developmental biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2009-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"34\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The International journal of developmental biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1387/ijdb.082659pc\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The International journal of developmental biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1387/ijdb.082659pc","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 34

摘要

果蝇单心(Sim)转录因子是中线发育过程中细胞命运的主要调控因子。同源小鼠Sim1和Sim2基因在中枢神经系统发育中起重要作用。mSim1活性的丧失导致下丘脑内特异性神经内分泌谱系的缺失,而mSim2的过表达导致行为缺陷。我们现在提供的证据表明,脊椎动物的Sim基因可能对肢体肌肉的形成很重要。我们通过原位杂交检测了Sim1和Sim2基因在鸡和小鼠胚胎肢体发育过程中的表达。两个Sim基因的表达主要与肢体肌肉的形成有关。我们发现,尽管Sim2基因在不同物种间存在一定差异,但在鸡和小鼠胚胎肢体中,每个Sim基因具有相似的时间和空间表达模式。在鸡或小鼠胚胎肢体中,Sim1和Sim2显示出不重叠的表达域,这表明Sim1和Sim2蛋白参与了肢体肌肉形成的不同步骤。在鸡和小鼠中,Sim1基因的表达与肌肉祖细胞迁移的早期阶段有关,而Sim2基因在迁移过程刚刚结束时表达。此外,鸡和小鼠的Sim2基因表达在肢体腹侧肌肉块中比在背侧腹侧肌肉块中增强。我们的结果为进一步分析Sim基因在肢体肌肉形成中的功能提供了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Sim1 and Sim2 expression during chick and mouse limb development.
The Drosophila Single minded (Sim) transcription factor is a master regulator of cell fate during midline development. The homolog mouse Sim1 and Sim2 genes are important for central nervous system development. Loss of mSim1 activity leads to an absence of specific neuroendocrine lineages within the hypothalamus, while overexpression of mSim2 leads to behavioural defects. We now provide evidence that vertebrate Sim genes might be important for limb muscle formation. We have examined by in situ hybridisation the expression of the Sim1 and Sim2 genes during limb development in chick and mouse embryos. The expression of both Sim genes is mainly associated with limb muscle formation. We found that each Sim gene has a similar temporal and spatial expression pattern in chick and mouse embryonic limbs, although with some differences for the Sim2 gene between species. In chick or mouse embryonic limbs, Sim1 and Sim2 display non-overlapping expression domains, suggesting an involvement for Sim1 and Sim2 proteins at different steps of limb muscle formation. Sim1 gene expression is associated with the early step of muscle progenitor cell migration in chick and mouse, while the Sim2 gene is expressed just after the migration process. In addition, chick and mouse Sim2 gene expression is enhanced in limb ventral muscle masses versus dorsal ventral muscle masses. Our results provide a basis for further functional analysis of the Sim genes in limb muscle formation.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信