蜜蜂白斑Apis蜂毒磷脂酶A2活性的研究

S. Topchiyeva, F. Mammadova
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引用次数: 0

摘要

本研究调查了阿塞拜疆不同地区蜜蜂(Apis mellifera caucasica l)毒液提取物的磷脂酶A2活性(在Baku, Sumgait, Sheki, Balaken, Zagatala, Shamakhi周边地区的蜂房)。采集毒液在春、夏、秋进行。用滴定法测定毒液中PLA2s的活性。在巴库郊区采集的毒液中,磷脂酶A2活性最低(134,7 IU/mg),在Zaqatala最高(183,7 IU/mg)。毒液中磷脂酶A2活性随季节的变化而变化。各地区蛇毒磷脂酶A2的平均活性均以夏季最高,秋季最低。实验数据的结果可用于确定蜂毒的生物活性鉴定和标准化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Study on Activity of Bee Venom Phospholipase A2 from Apis mellifera caucasia L in Honey Bees
This study investigates of phospholipase A2 activity in venom extracts from honey bees (Apis mellifera caucasica l) from different regions of Azerbaijan (on apiaries of surrounding area of Baku, Sumgait, Sheki, Balaken, Zagatala, Shamakhi). Collection of venom carried out in the spring, summer, autumn. The PLA2s activity in the venom was determined the titration method. The lowest phospholipase A2 activity was found in venom collected in suburb of Baku (134, 7 IU/mg), and highest in Zaqatala (183, 7 IU/mg). Phospholipase A2 activity was obtained in venom with respect to season of the year. The highest mean activity of phospholipase A2 in venom was found in summer season, the lowest activity is during autumn in all regions. The results of experimental data can be used for determining the biological activity of identification and standardization of honey bee venom.
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