F. F. Cassana, A. R. Falqueto, E. Braga, J. Peters, M. A. Bacarin
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引用次数: 16
摘要
在含20和40 g L-1蔗糖的Murashige和Skoog (MS)培养基上，在不同光子通量密度(21和60µmol m-2 s-1)下培养甘薯(Ipomoea batata L.)植株。离体发育的叶片大多表现出较高的可变最大荧光比(FV/FM)，表明光合机构发育良好。随着叶片老化，特别是在高蔗糖浓度和高光子通量培养基上离体培养的植株，这一比率下降。在整个试验过程中，离体发育的叶片均表现出较高的FV/FM比率。有效光化学效率(FV'/FM')在叶片出芽后第15天达到最大值。离体发育叶片的光合势速率高于离体起源叶片。
Chlorophyll a fluorescence of sweet potato plants cultivated in vitro and during ex vitro acclimatization
Sweet potato (Ipomoea batatas L.) plants were cultivated in vitro in Murashige and Skoog (MS) medium with 20 and 40 g L-1 of sucrose under two different photon flux densities (21 and 60 µmol m-2 s-1). Leaves developed in vitro mostly showed high variable to maximum fluorescence ratio (FV/FM) , indicating good development of photosynthetic apparatus. This ratio decreased during leaf aging, especially in the plants cultivated in vitro on medium with higher sucrose concentration and higher photon flux. Leaves developed ex vitro showed high FV/FM ratio during whole experiment. The effective photochemical efficiency (FV'/FM') was maximum at 15th day after emergence of leaves. Photosynthetic potential rate was higher in leaves developed in vitro than in leaves originated ex vitro.
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