Isolation and Medium Optimization of Bacterial Lipase from oil Mill

Lipase (glycerol ester hydrolases EC 3,1,1,3) is a important biocatalyst because of their demand in various industrial application. In this study lipolytic bacteria isolate KK12 Bacillus spp. was isolate from oil contaminated sample by using tributyrine agar medium. Isolate KK12 bacillus spp gave the highest zone of hydrolysis with 25mm. Lipase production was improved by optimized for different oils, carbon sources, nitrogen sources, substrate, pH, temperature, metal ions. The highest lipase activity was 14U/l at 37 0 C at pH 7.6. In this study isolate KK12 Bacillus spp giving best activity with peptone was 9.2U/l. Lipase activity was high in olive oil about 9.8U/l at 37 0 C after 48hours incubation than other substrate. Other different parameters like pH, temp., metal ions also study in this research same all of these.