Formulation of Moisturizing Cream Containing Collagen and Argan Oil: An in vivo Evaluation

Introduction: Natural oils, including argan oil, are used to moisturize the skin. Collagen is one of the body's proteins that plays an important role in the structure of the skin. Therefore, in this study, the moisturizing effect of creams containing collagen and argan oil was studied. Method: Using Design Expert statistical software, 13 formulations were designed and synthesized. They were evaluated for spreadability and viscosity, and the optimized formulation was selected. The formulations underwent physicochemical control experiments, such as uniformity of products, thermal stress, centrifugal force resistance, and pH measurements. The moisturizing effect was investigated with in-vitro and in-vivo studies. Results: Cream containing 5% collagen and 5% argan oil was selected as the optimal formulation. The blocking factor of the cream containing collagen and argan oil was higher than the negative control 48 h after the study (P<0.05). The amount of hydration of the cream containing collagen and argan oil on the skin of the hand was higher than the negative control 5 h after the study (P<0.05). The optimal formulation showed good stability against physicochemical control experiments. Conclusion: Moisturizing cream containing collagen and argan oil had a more moisturizing effect than cream without collagen and argan oil and increased skin hydration