牛蒡子ITS序列与牛蒡子品质的相关性研究

Q3 Pharmacology, Toxicology and Pharmaceutics

Zhongguo Zhongyao Zazhi Pub Date : 2011-07-01 DOI:10.4268/CJCMM20111419

Liang Xu, D. Dou, B. Wang, Yan-yun Yang, Tingguo Kang

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引用次数: 0

摘要

目的研究牛蒡子ITS序列与不同产地牛蒡子品质的相关性。方法从26个不同产地采集牛蒡子药材样品。采用聚合酶链反应(PCR)测定其ITS序列，高效液相色谱法测定牛蒡苷含量，评价其质量。采用ClustalX(1.81)、Mage 4.0、SPSS 13.0统计软件进行遗传多样性、基因型及相关性分析。结果从26份样本中获得A.菌株序列，并在GenBank中登记。测定牛蒡子中相应的牛蒡素含量和千粒重。经统计分析，牛蒡子基因型与牛蒡子品质相关。结论本研究为揭示牛蒡地药学中药的分子机制提供了基础。

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[Study on correlation between ITS sequence of Arctium lappa and quality of Fructus Arctii].

OBJECTIVE To study the correlation between ITS sequence of Arctium lappa and Fructus Arctii quality of different origin. METHOD The samples of Fructu arctii materials were collected from 26 different producing areas. Their ITS sequence were determined after polymerase chain reaction (PCR) and quality were evaluated through the determination of arctiin content by HPLC. Genetic diversity, genotype and correlation were analyzed by ClustalX (1.81), Mage 4.0, SPSS 13.0 statistical software. RESULT ITS sequence of A. was obtained from 26 samples, and was registered in the GenBank. Corresponding arctiin content of Fructus arctii and 1000-grain weight were determined. A. lappa genotype correlated with Fructus arctii quality by statistical analysis. CONCLUSION The research provided a foundation for revealing the molecular mechanism of Fructus arctii geoherbs.

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来源期刊

Zhongguo Zhongyao Zazhi Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

1.50

自引率

0.00%

发文量

581

期刊介绍：