In Vitro Plant Regeneration of Sugarcane (Saccharum spp.) Variety Inoculated Under Different Levels of Plant Growth Regulators

Sugarcane (Saccharum spp.) is an excellent candidate for mass propagation using tissue culture as seed multiplication using the conventional method is time consuming. The main objective of this study was to optimize an efficient and reliable protocol for direct propagation of sugarcane varieties under in vitro condition. Different levels of BAP along with kinetin were used for shoot proliferation. The proliferated shoots were subjected to MS medium supplemented with different levels of NAA. Results of the present study revealed both the BAP and Kinetin had a synergistic effect and none of them generated the maximum response while separately applied. The maximum number of shoots (10.3 ± 0.31), shoot length (3.77 ± 0.40) and number of leaves (3.87 ± 0.17) were recorded on the MS medium supplemented with 2 mg/l of BAP and 1.5 mg/L of Kinetin. In the rooting medium, highest rooting frequency (100%), root length (2.21 cm) and number of roots per shoots (20.13) were recorded on the half strength MS semi-solid media fortified with 5 mg/L NAA. The better rooted plantlets were transferred to green house for hardening under pot comprised of sand, soil and farm yard manure in 1:1:1 ratio and 100% of the well rooted plantlets survived under greenhouse conditions.