一种测定固定在酶电极中的葡萄糖氧化酶量的测定方法

J. Gooding, M. Situmorang, P. Erokhin, D. B. Hibbert
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引用次数: 34

摘要

本文提出了一种简单的测定固定在酶电极表面葡萄糖氧化酶量的方法。用8 M尿素溶液从固定化葡萄糖氧化酶中剥离两个黄素腺嘌呤二核苷酸(FAD)辅因子。用荧光或伏安法定量测定尿素溶液中FAD的浓度,以确定电极表面葡萄糖氧化酶的量。该分析适用于各种不同的酶电极,并使用石英晶体微天平对单层酶电极进行验证。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
An assay for the determination of the amount of glucose oxidase immobilised in an enzyme electrode
This paper presents a simple assay for the estimation of the amount of glucose oxidase immobilised onto the surface of enzyme electrodes. The two flavin adenine dinucleotide (FAD) cofactors were stripped from immobilised glucose oxidase using an 8 M urea solution. The resultant concentration of FAD in the urea solution was quantified using either fluorescence or voltammetry to determine the amount of glucose oxidase on the electrode surface. The assay is applied to a variety of different enzyme electrodes and is verified for a monolayer enzyme electrode using a quartz crystal microbalance.
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