尼日利亚本土和当地适应的外来火鸡品种肌肉生长抑制素基因位点的单核苷酸多态性和遗传多样性评价

Nigerian Journal of Biotechnology Pub Date : 2022-08-03 DOI:10.4314/njb.v39i1.3

B. Ilori, S. Isong, E. B. Oghate, B. Ojeniyi, So Durosaro, K. Akano, D. Oguntade, B.M. Onakoya, S.A. Olapade, M. Ozoje

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引用次数: 0

摘要

本研究对本地和尼日利亚本地适应的外来火鸡肌肉生长抑制素基因位点的单核苷酸多态性进行了表征。总共220日龄的家禽，包括120只本地火鸡和100只当地适应的外来火鸡，来自信誉良好的孵化场，并在集约化管理系统下进行了20周的管理。在火鸡8周龄时，分别从这两个品种的70只火鸡身上采集血样，经臂静脉注入乙二胺四乙酸(EDTA)瓶中，使用市售试剂盒进行DNA提取和靶区扩增。利用BioEdit、Codon Code Aligner、DnaSP和MEGA软件对含有MSTN基因外显子1和2及其周围内含子的基因组区域进行测序和分析。还进行了基于密码子的测试，以估计非同义替换(dN)与同义替换(dS)的比例。结果表明，本地和外来火鸡品种在第1外显子均存在一个非同义单核苷酸多态性(SNP) (248 G>A)，而外来火鸡品种仅在第2外显子中检测到SNP变异333 G>A。在内含子1中，共检测到3个本地火鸡snp和2个外来火鸡snp。内含子2也分别在本地火鸡和外来火鸡中发现了4个和3个snp。遗传多样性指数表明，本地火鸡在内含子1和内含子2的单倍型多样性较高(75%)，而外来火鸡在外显子2的单倍型多样性估计为20%。我们基于密码子的选择测试显示，G248A SNP位点的dN/dS比<1(纯化选择)，表明这种非同义SNP变体可能对生长性能起作用。我们建议开展一项基于种群的研究，以调查这种非同义SNP G248A对生长和形态结构性状的影响，从而改善和保护我们当地适应的火鸡种群。

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Evaluation of Single Nucleotide Polymorphism and Genetic Diversity at the Myostatin gene Locus in Indigenous and Locally Adapted Exotic Turkey breeds in Nigeria

This research was carried out to characterize single nucleotide polymorphism at the myostatin gene locus in indigenous and Nigerian locally adapted exotic turkeys. A total of 220-day-old poults comprising 120 local and 100 locally adapted exotic turkeys were sourced from reputable hatchery and were managed for 20 weeks under intensive management system. Blood samples were collected from 70 turkeys each form the two breeds via the brachial vein into Ethylene Diamine Tetra Acetic Acid (EDTA) bottles for DNA extraction and amplification of target regions using commercially available kits when the birds were 8 weeks. Genomic regions containing exon 1 and 2 of MSTN gene including their surrounding introns were sequenced and analyzed using BioEdit, Codon Code Aligner, DnaSP and MEGA software. Codon-based test was also performed to estimate the ratio of non-synonymous substitutions (dN) to synonymous substitutions (dS). Results from our study showed that both local and exotic turkey breeds had one non-synonymous single nucleotide polymorphism (SNP) (248 G>A) in exon 1 while SNP variant 333 G>A was detected only in exon 2 of exotic turkey. In intron 1, a total of 3 SNPs in local turkey and 2 SNPs in exotic turkey were detected. Intron 2 also revealed 4 and 3 SNPs in local and exotic turkeys respectively. Genetic diversity indices showed that local turkey had higher haplotype diversity at intron 1 (75 %) and intron 2 (84 %) while haplotype diversity of 20 % was estimated at exon 2 in exotic turkey. Our Codon-based test of selection showed dN/dS ratio of <1 (purifying selection) at G248A SNP loci, suggested a possible role of this non-synonymous SNP variant on growth performance. We recommend a population-based study to investigate the effect of this non-synonymous SNP G248A on growth and morpho-structural traits for breed improvement and conservation of our locally adapted turkey populations.

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Nigerian Journal of Biotechnology

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