琼脂糖中白细胞迁移试验。使用嘌呤霉素揭露非免疫抑制。

Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology Pub Date : 2009-08-15 DOI:10.1111/J.1699-0463.1974.TB02323.X

K. Mygind, S. Stenbjerg

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引用次数: 5

摘要

通过琼脂糖培养基白细胞迁移试验，研究了人外周血白细胞对PPD和结核杆菌的反应。在PPD 50 μg/ml时，结核菌素阳性者白细胞的迁移均受到抑制，而结核菌素阴性者白细胞的迁移未见抑制。两组心肌梗死无重叠。在PPD为50 μg/ml和100 μg/ml时，嘌呤霉素阻断蛋白合成可阻止结核菌素阳性细胞的迁移抑制。在某些情况下，结核菌素阴性者细胞的迁移在PPD 100 μg/ml的存在下受到抑制。这种抑制不能被嘌呤霉素阻止，可能是由于PPD的毒性作用。提示嘌呤霉素可用于脱臼、假的、可能有毒的抑制。

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Leucocyte migration test in agarose. The use of puromycin in disclosure of non-immunological inhibition.

The response of peripheral human leucocytes to PPD and tubercle bacilli was studied in the leucocyte migration test in agarose medium. At PPD 50 μg/ml migration of leucocytes from tuberculin positive persons was inhibited in all cases tested, while no migration inhibition of cells from tuberculin negative persons was found. There was no overlapping between the MI of the two groups. Blocking of protein synthesis by means of puromycin prevented the migration inhibition of cells from tuberculin positive persons at PPD 50 μg/ml and 100 μg/ml. In some cases the migration of cells from tuberculin negative persons was inhibited in the presence of PPD 100 μg/ml. This inhibition could not be prevented by puromycin and was probably due to a toxic effect of PPD. It is suggested that puromycin may be useful in dislocating false, probably toxic, inhibition.

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Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology

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