在自然种群中鉴定银齿羚杂交品种(人齿羚和博纳齿羚)的诊断位点

IF 0.5 4区农林科学 Q4 FISHERIES

Boletim do Instituto de Pesca Pub Date : 2022-01-01 DOI:10.20950/1678-2305/bip.2022.48.e684

Daiane Machado Souza, Suzane Fonseca Freitas, R. Tavares, H. Moreira

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引用次数: 0

摘要

杂交是一种自然现象，在鱼类中比在其他脊椎动物中更常见。核和线粒体分子标记的使用为这些事件的检测提供了有价值的结果。本研究的目的是研究银菜自然居群中种间杂交的发生情况。在巴西南部Mangueira泻湖采集了人类齿齿蜥、博纳利齿齿蜥和形态上与纯种不同的个体标本。结果:合成并检测了6个四核苷酸微卫星位点。结果表明，UFPEL_OH3基因座对银侧杂交的检测具有诊断性，可以区分纯种和杂交种。从GenBank遗传数据库中保守的齿鲨序列合成的线粒体标记基因cytb显示，样本的基因序列没有差异，需要进一步的研究来证实这一假设。

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Diagnostic locus for identification of silverside hybrids (odontesthes humensis and odontesthes bonariensis) in a natural population

Hybridization is a natural phenomenon that occurs more often in fish than in other vertebrates. The use of nuclear and mitochondrial molecular markers provides valuable results in the detection of these events. The aim of this study was to investigate the occurrence of interspecific hybrids in natural populations of silverside. The samples of Odontesthes humensis, Odontesthes bonariensis, and individuals that were morphologically different from pure species were collected in the Mangueira lagoon, located in southern Brazil. Result: Six tetranucleotide microsatellite loci were synthesized and tested. The UFPEL_OH3 locus proved to be diagnostic for the detection of silverside hybrids, and it was possible to distinguish between pure and hybrid species. The mitochondrial marker gene cytb synthesized from conserved Odontesthes sequences in the GenBank genetic database showed no differences in the genetic sequence of the samples, needing further studies to confirm the hypothesis.

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来源期刊

Boletim do Instituto de Pesca FISHERIES-ZOOLOGY

CiteScore

0.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： To publish original articles of research and short communications in the following áreas: Fisheries, Aquaculture, Zootechnology, Limnology, Oceanography, Biology and Pathology of aquatic organisms. The publication depends on the approval of the Editorial Board, based on the peer review.