植物生长调节剂、蔗糖和花节位置对兰州百合微繁和鳞茎生长的影响

2011 International Conference on Remote Sensing, Environment and Transportation Engineering Pub Date : 2011-07-29 DOI:10.1109/RSETE.2011.5964101

Tao Zhang, Jing-long Liang, Yan Zou, Ron-chong Li

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引用次数: 0

摘要

研究了兰州百合(Lilium davidii)的微繁技术方案。在添加了0.5 mg·L−1 NAA + 0.5 mg·L−1 BAP和6-9%蔗糖的MS培养基上，切除鳞片基部的鳞茎形成、平均每个外植体鳞茎数、鲜重和其他鳞茎再生参数的效果最好。在含0.1 mg·L−1 NAA的半强MS培养基上100%生根。生根后，克隆苗成功地硬化到离体条件，95%的再生苗在土壤中正常成活。

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Micropropagation and bulblet growth of Lanzhou lily affected by plant growth regulators, sucrose and segments position

A protocol for micropropagation of Lanzhou lily (Lilium davidii), an edible and ornamental plant in China was developed. The best results for bulblet formation, mean number of bulblets per explant, fresh weight and other bulblet regeneration parameters were obtained on the basal portion of the excised bulbscales on MS medium supplemented with 0.5 mg·L⁻¹ NAA + 0.5 mg·L⁻¹ BAP and 6–9% sucrose. 100% shoots were rooted on half-strength MS media containing 0.1 mg·L⁻¹ NAA. After rooting the cloned plantlets were successfully hardened to ex vitro conditions and 95% of the regenerated plantlets survived normally in the soil.

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2011 International Conference on Remote Sensing, Environment and Transportation Engineering

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