Preliminary secondary metabolites detection of aqueous extracts obtained from different tissues of in-vitro originated Eclipta alba

Eclipta alba is well known herbal plant for its vast range of pharmaceutically important properties. Some of its major activities are antimytotoxic, antimicrobial, antihepatotoxic, antioxidant, antihyperglycemic, rejuveniser and antivenom action. The present study reveals the presence of secondary metabolites responsible for imparting pharmacological properties in different parts like apical tissue, meristematic cells, whole plant, Callus, Seedlings, Mature seeds, immature seeds and dries seeds obtained via plant tissue culture technique. The results shows the absence of Alkaloids in tissue cultures plants which has been normally reported to be present in huge amounts in naturally occurring plants in aqueous extracts but other metabolites viz., Flavonoids, Tannins and phenolic groups reported earlier to be occurring in Eclipta alba and its parts were found to be present in aqueous extracts of tissue cultured plant.