剑花中黄酮类化合物的提取及其制备方法

Liu Chongbi, Xu Mingying, C. Ying, C. Ling, Dong Shumei
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引用次数: 0

摘要

七星花(Hylocerei Undati Flos)不仅是肇庆特产植物,而且因其含有多种有效成分而备受关注。为探究七星剑花中总黄酮的作用,通过设计单因素试验和L9(4次3次幂)正交试验,研究了不同提取时间、乙醇浓度和粒径对总黄酮得率的影响,并对工艺进行了优化。经超声和95%乙醇提取后,再进行乙酸乙酯提取、薄层析等鉴定。采用水杨酸法和抗菌试验对提取物进行抗氧化能力分析。然后以剑花醇提取物为原料制备了保湿抗氧化霜。结果表明,在乙醇浓度为95%、料液比为1:60、提取时间为60 min、提取温度为70℃的条件下,提取率最高,达13.41 mg/g。提取物抑菌作用明显,对羟基自由基有明显的清除作用。因此,本文对今后黄酮类化合物的进一步研究和应用具有重要的理论和现实意义。可以预见,肇庆中药七星剑花中提取的黄酮类化合物,未来将广泛应用于食品、医药、保健等领域。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Extraction of Flavonoids from Sword Flower and Methodology of Its Preparations
Qixing Jianhua (Hylocerei Undati Flos) is not only a specialty plant of Zhaoqing, but also has a lot of attention because it contains a variety of active ingredients. In order to explore the role of flavonoids in the seven-star sword-flower, by designing single factor test and L9 (Fourth power of 3) orthogonal test, the effects of different extraction time, ethanol concentration and particle size on the total flavonoids yield and process optimization were studied. After ultrasonic and 95% alcohol extraction, followed by ethyl acetate extraction, thin plate chromatography and other identification. Antioxidant ability analysis of the extract was carried out by salicylic acid method and antimicrobial test. And then the moisturizing anti-oxidation cream was prepared by using sword-flower alcohol extract. The results showed that the extraction rate was highest at 95% ethanol concentration, 1:60 of material to liquid ratio, 60 min extraction time, and 70 degrees Celsius extraction temperature, reaching 13.41 mg/g. The antibacterial effect of the extract is obvious, and it has obvious scavenging effect of hydroxyl radicals. Therefore, this paper has important theoretical and practical significance for the further study and application of flavonoids in the future. It can be predicted that the flavonoids extracted from Qixing sword flower, a kind of traditional Chinese medicine in Zhaoqing, will be widely used in the fields of food, medicine and health in the future.
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