烟叶固化过程中可溶性蛋白的电泳图谱

Q3 Agricultural and Biological Sciences
S. J. Sheen, B.I. Townes
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引用次数: 2

摘要

摘要聚丙烯酰胺凝胶圆盘电泳在白肋、土耳其、烟熏和烤烟7种烟草株系的叶片提取物中发现了至少13个阳极蛋白带。B、E、G、J 4个主要谱带在所有菌株中均存在。弱带在烟叶类型间的活动性和数量略有不同。对白肋21号叶片细胞组分的研究表明,A、C、D、G和J波段存在于细胞质中,而除了B、E、L和M波段外,其他波段在叶绿体、线粒体和核提取物中似乎都是相同的。细胞器间蛋白质带带模式的相似性表明它们的膜蛋白具有相似性。带B、E、L和M与质膜或细胞壁有关。在模拟空气固化过程中,大部分波段在第10天减弱,而波段B、E和G在固化叶片中保持较高强度,波段C的浓度有所降低。波段B、E和G与周期性酸性希夫试剂(PAS)反应呈阳性,但与胶体离子染色反应呈阴性。胰蛋白酶消化使氨基黑或PAS试剂染色的蛋白带消失。结果表明,B、E和G为中性或弱酸性糖蛋白
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Electrophoretic Patterns of Soluble Proteins in Tobacco Leaf During Curing
Abstract Polyacrylamide gel disc electrophoresis revealed at least 13 anodic protein bands in leaf extracts of seven tobacco (Nicotiana tabacum L.) strains representing Burley, Turkish, flue- and fire-cured types. Four major bands B, E, G, and J were common in all strains. Weak bands varied slightly in mobility and in number among tobacco types. Studies of cell fractions of Burley 21 leaves yielded information that bands A, C, D, G, and J are present in the cytoplasm, whereas all but bands B, E, L, and M appeared to be identical in the chloroplast, mitochondrial, and nuclear extracts. The resemblance in protein banding pattern among the organelles indicates similarity in their membrane proteins. Bands B, E, L, and M are associated with plasma membrane or cell wall. During simulated air-curing, a majority of bands diminished by the tenth day, whereas bands B, E, and G remained highly intense and the concentration of band C was reduced in cured leaves. Bands B, E, and G reacted positively with the periodic-acid Schiff's reagent (PAS) but negatively with the colloidal ion staining. Trypsin digestion abolished protein bands stained with amido black or the PAS reagent. Results suggest that bands B, E, and G are neutral or weakly acidic glycoproteins
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来源期刊
Beitrage zur Tabakforschung International/ Contributions to Tobacco Research
Beitrage zur Tabakforschung International/ Contributions to Tobacco Research Agricultural and Biological Sciences-Agronomy and Crop Science
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