鲎血青素亚基IIIa、IIIb和IV的结晶学初步研究

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology Pub Date : 2002-04-29 DOI:10.1016/S0167-4838(02)00231-5

Shenping Liu , Karen A. Magnus

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引用次数: 2

摘要

鲎血青素亚基IIIa、IIIb和IV的晶体适合x射线衍射分析。亚基IV的三维结构由分子置换法和非晶体对称平均法确定。从部分数据集得到了一个亚单位IIIa的暂定模型。这两种结构都与亚基II相似，可以提供适合于寡核苷酸探针合成的一级结构片段。

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Preliminary crystallographic studies of Limulus polyphemus hemocyanin subunits IIIa, IIIb and IV

Crystals of Limulus hemocyanin subunits IIIa, IIIb and IV are suitable for X-ray diffraction analysis. The three-dimensional structure of subunit IV is determined by molecular replacement and non-crystallographic symmetry averaging methods. A tentative model of subunit IIIa is obtained from a partial data set. Both structures, similar to subunit II, could provide primary structure segments suitable for oligonucleotide probe synthesis.

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Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology

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