{"title":"头孢酞素可减轻啮齿动物肢体缺血再灌注模型的肝损伤","authors":"Yin-Kuang Chang , Su-Cheng Huang , Ming-Chang Kao , Chun-Jen Huang","doi":"10.1016/j.aat.2015.11.004","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><p>Limb ischemia–reperfusion (I/R) causes remote organ injury (e.g., liver injury). Oxidation and inflammation are crucial mechanisms. We investigated the effects of cepharanthine, a potent antioxidative and anti-inflammatory drug, on alleviating liver injury induced by limb I/R.</p></div><div><h3>Methods</h3><p>Twenty-four adult male Sprague-Dawley rats were randomized to receive sham operation (Sham), Sham plus cepharanthine, I/R, or I/R plus cepharanthine and designated as the Sham, Sham+Cep, I/R, or I/R+Cep group, respectively (<em>n</em> = 6 in each group). I/R was induced by applying rubber band tourniquets high around each hind limb for 3 hours followed by reperfusion for 24 hours.</p></div><div><h3>Results</h3><p>The plasma levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) of the Sham and Sham+Cep groups were low, and the levels of AST and ALT of the I/R group were significantly higher than those of the Sham group (both <em>p</em> <!--><<!--> <!-->0.001). By contrast, the AST and ALT of the I/R+Cep group were significantly lower than those of the I/R group (both <em>p</em> <!--><<!--> <!-->0.001). The hepatic levels of nitric oxide (NO), malondialdehyde (MDA), macrophage inflammatory protein 2 (MIP-2), interleukin-6 (IL-6), and cyclooxygenase-2 (COX-2)/prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) of the Sham and Sham+Cep groups were also low. As expected, the NO, MDA, MIP-2, IL-6, and COX-2/PGE<sub>2</sub> of the I/R group were significantly higher than those of the Sham group (all <em>p</em> <!--><<!--> <!-->0.001). By contrast, the NO, MDA, MIP-2, IL-6, and COX-2/PGE<sub>2</sub> of the I/R+Cep group were significantly lower than those of the I/R group (all <em>p</em> <!--><<!--> <!-->0.05).</p></div><div><h3>Conclusion</h3><p>Cepharanthine alleviates liver injury in a rodent model of limb I/R. The mechanisms may involve reducing oxidation and inflammation.</p></div>","PeriodicalId":87042,"journal":{"name":"Acta anaesthesiologica Taiwanica : official journal of the Taiwan Society of Anesthesiologists","volume":"54 1","pages":"Pages 11-15"},"PeriodicalIF":0.0000,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.aat.2015.11.004","citationCount":"12","resultStr":"{\"title\":\"Cepharanthine alleviates liver injury in a rodent model of limb ischemia–reperfusion\",\"authors\":\"Yin-Kuang Chang , Su-Cheng Huang , Ming-Chang Kao , Chun-Jen Huang\",\"doi\":\"10.1016/j.aat.2015.11.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><p>Limb ischemia–reperfusion (I/R) causes remote organ injury (e.g., liver injury). Oxidation and inflammation are crucial mechanisms. We investigated the effects of cepharanthine, a potent antioxidative and anti-inflammatory drug, on alleviating liver injury induced by limb I/R.</p></div><div><h3>Methods</h3><p>Twenty-four adult male Sprague-Dawley rats were randomized to receive sham operation (Sham), Sham plus cepharanthine, I/R, or I/R plus cepharanthine and designated as the Sham, Sham+Cep, I/R, or I/R+Cep group, respectively (<em>n</em> = 6 in each group). I/R was induced by applying rubber band tourniquets high around each hind limb for 3 hours followed by reperfusion for 24 hours.</p></div><div><h3>Results</h3><p>The plasma levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) of the Sham and Sham+Cep groups were low, and the levels of AST and ALT of the I/R group were significantly higher than those of the Sham group (both <em>p</em> <!--><<!--> <!-->0.001). By contrast, the AST and ALT of the I/R+Cep group were significantly lower than those of the I/R group (both <em>p</em> <!--><<!--> <!-->0.001). The hepatic levels of nitric oxide (NO), malondialdehyde (MDA), macrophage inflammatory protein 2 (MIP-2), interleukin-6 (IL-6), and cyclooxygenase-2 (COX-2)/prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) of the Sham and Sham+Cep groups were also low. As expected, the NO, MDA, MIP-2, IL-6, and COX-2/PGE<sub>2</sub> of the I/R group were significantly higher than those of the Sham group (all <em>p</em> <!--><<!--> <!-->0.001). By contrast, the NO, MDA, MIP-2, IL-6, and COX-2/PGE<sub>2</sub> of the I/R+Cep group were significantly lower than those of the I/R group (all <em>p</em> <!--><<!--> <!-->0.05).</p></div><div><h3>Conclusion</h3><p>Cepharanthine alleviates liver injury in a rodent model of limb I/R. The mechanisms may involve reducing oxidation and inflammation.</p></div>\",\"PeriodicalId\":87042,\"journal\":{\"name\":\"Acta anaesthesiologica Taiwanica : official journal of the Taiwan Society of Anesthesiologists\",\"volume\":\"54 1\",\"pages\":\"Pages 11-15\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.aat.2015.11.004\",\"citationCount\":\"12\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta anaesthesiologica Taiwanica : official journal of the Taiwan Society of Anesthesiologists\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1875459715300187\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta anaesthesiologica Taiwanica : official journal of the Taiwan Society of Anesthesiologists","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1875459715300187","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Cepharanthine alleviates liver injury in a rodent model of limb ischemia–reperfusion
Objectives
Limb ischemia–reperfusion (I/R) causes remote organ injury (e.g., liver injury). Oxidation and inflammation are crucial mechanisms. We investigated the effects of cepharanthine, a potent antioxidative and anti-inflammatory drug, on alleviating liver injury induced by limb I/R.
Methods
Twenty-four adult male Sprague-Dawley rats were randomized to receive sham operation (Sham), Sham plus cepharanthine, I/R, or I/R plus cepharanthine and designated as the Sham, Sham+Cep, I/R, or I/R+Cep group, respectively (n = 6 in each group). I/R was induced by applying rubber band tourniquets high around each hind limb for 3 hours followed by reperfusion for 24 hours.
Results
The plasma levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) of the Sham and Sham+Cep groups were low, and the levels of AST and ALT of the I/R group were significantly higher than those of the Sham group (both p < 0.001). By contrast, the AST and ALT of the I/R+Cep group were significantly lower than those of the I/R group (both p < 0.001). The hepatic levels of nitric oxide (NO), malondialdehyde (MDA), macrophage inflammatory protein 2 (MIP-2), interleukin-6 (IL-6), and cyclooxygenase-2 (COX-2)/prostaglandin E2 (PGE2) of the Sham and Sham+Cep groups were also low. As expected, the NO, MDA, MIP-2, IL-6, and COX-2/PGE2 of the I/R group were significantly higher than those of the Sham group (all p < 0.001). By contrast, the NO, MDA, MIP-2, IL-6, and COX-2/PGE2 of the I/R+Cep group were significantly lower than those of the I/R group (all p < 0.05).
Conclusion
Cepharanthine alleviates liver injury in a rodent model of limb I/R. The mechanisms may involve reducing oxidation and inflammation.
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