利用skl标记的DsRed蛋白可视化大孢子索达菌的过氧化物酶体

Fungal Genetics Reports Pub Date : 2008-12-01 DOI:10.4148/1941-4765.1083

Skander Elleuche, S. Pöggeler

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引用次数: 32

摘要

我们报道了利用盘状虫红色荧光蛋白DsRed来观察丝状子囊菌大孢子索达尔的过氧化物酶体。为了实现过氧化物酶体的标记，将DsRed与丝氨酸-赖氨酸-亮氨酸标签(SKL)融合。在细粒曲霉gpd启动子调控下，DsRed- skl融合基因的表达导致DsRed蛋白导入过氧化物酶体。本文介绍了我们在构建pDsRed-SKL载体及其应用方面的研究成果。

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Visualization of peroxisomes via SKL-tagged DsRed protein in Sordaria macrospora

We report the utilization of Discosoma sp. red fluorescent protein DsRed to visualize peroxisomes in the filamentous ascomycete Sordaria macrospora. To achieve labeling of peroxisomes, DsRed was fused to a serine-lysine-leucine tag (SKL). Expression of the DsRed-SKL fusion gene under the control of the Aspergillus nidulans gpd-promoter led to protein import of DsRed into peroxisomes. In this study, we describe our results concerning the construction as well as the application of vector pDsRed-SKL.

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Fungal Genetics Reports

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