根据不同浓度PEG-6000溶液的抗旱性对全国小扁豆品种进行分化

N. Vus, A. О. Vasylenko, L. Shevchenko
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引用次数: 0

摘要

的目标。在PEG-6000溶液上对国家收集的扁豆的抗旱性水平进行评估。方法。在t = 21℃的滤纸床上进行生长板萌发,培养5天。采用PEG-6000工作溶液,浓度分别为15.0%和19.5%。对照在蒸馏水中发芽。测定了茎、根的长度,计算了生长过程的抑制程度。结果。在浓度为19.5%的PEG-6000溶液中萌发时,绝大多数材料的茎部和根部的抑制指标均在85%以上,60%以上,因此无法分离出耐PEG-6000作用的样品。当在15% PEG-6000溶液上发芽时,变异范围更大:“根长”特征的下降范围为0%至100%,“枝长”特征的下降范围为50%至100%。计算出的斯皮尔曼等级相关系数证实,在两种不同的实验中,与根相比,芽对渗透作用更敏感。结论。建立了PEG-6000的浓度,用于来源选择和抗旱性排序。从所研究的样本资料中确定了抗旱性的来源。微精子:8-MIL m5 (UD0600007,法国)、UD0600028(埃塞俄比亚)、Giza 9 (UD0600065,埃及),抗旱性加入标准为Gornostepnaya (UD0600131,亚美尼亚)亚种。microsperma。关键词:扁豆,PEG-6000,浓度,抗旱性来源
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Differentiation of accessions from the national lentil collection according to the level of drought resistance on a various concentrations PEG-6000 solution
Aim. assessment  of  accessions from the lentil national collectionon the level of drought resistance on a PEG-6000 solution. Methods. The germination was carried out in growth plates on a filter paper bed at t = 21ºC for five days. A working solution of PEG-6000 with concentrations of 15.0% and 19.5% was used. The control was germinated in distilled water. The length of the shoot and root was measured and the level of depression of growth processes was calculated. Results. When germinating on a PEG-6000 solution with 19.5% concentration, the overwhelming number of accessions had depression indicators for shoots above 85% and above 60% for roots, and therefore it was not possible to isolate samples tolerant to the action of PEG-6000. When germinating on a 15%  solution of PEG-6000, a wider range of variation was noted: depression in feature of “root length” ranged from 0% to 100%, “shoot length” - from 50% to 100%. The calculated Spearman's rank correlation coefficient confirms that shoots, in comparison with roots, are more sensitive to the action of osmotic in both variants of the experiment. Conclusions. The concentration of PEG-6000 was established for the selection of sources and the ranking of samples by drought resistance. Sources of drought resistance were identified from the studied sample – accessions subsp. microsperma: 8-MIL M 5 (UD0600007, France), UD0600028 (Ethiopia), Giza 9 (UD0600065, Egypt) and the accession-standard of drought resistance is Gornostepnaya (UD0600131, Armenia) subsp. microsperma. Keywords: lentil, PEG-6000, concentration, sources of drought resistance.
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