{"title":"Antifilarial Activity of Cucurbita pepo ovifera var ovifera (Cucurbitaceae) on Onchocerca ochengi Adult Worms","authors":"Justin Kalmobé, D. Ndjonka, J. Dikti, E. Liebau","doi":"10.9734/BJPR/2017/33381","DOIUrl":null,"url":null,"abstract":"One of the strategies for developing novel pharmaceutical drugs is to use natural sources such as plants for therapeutic treatment. Plant extracts are a cocktail of compounds which act synergically and can improve treatment effectiveness, reduce therapeutic duration and resistance. The ethanolic extracts of leaves and seeds of Cucurbita pepo ovifera var ovifera from Sudano-Guinean and Sudano-Sahelian zones of Cameroon were evaluated on the cattle parasite nematode Onchocerca ochengi. Worms were incubated with different concentrations of the plant extracts in RPMI-1640 supplemented with streptomycin and gentamicin. Mortality at 37°C was monitored after 24, 48 and 72 h. Ivermectin was used as positive control and DMSO as negative. Plant extracts Original Research Article Kalmobé et al.; BJPR, 17(2): 1-8, 2017; Article no.BJPR.33381 2 from the two ecological zones showed anthelminthic activities on O. ochengi after 72 h with LC50 varying from 20 to 1090 μg /mL. The highest antifilarial activity in Sudano-Guinean zone was obtained with leave extract of C. pepo ovifera (LC50 of 20 μg/mL), while highest antifilarial activity in Sudano-Sahelian zone was obtained with seed extracts of the plant with LC50 value of 17 μg/mL after 72 h. These results show that anthelmintic activity depends on the part of the plant and the ecological zones. Additionally, the plant is not toxic. These results on the ethanolic extracts of leaves and seeds of C. pepo ovifera var ovifera confirmed the use of this plant in traditional medicine in Cameroon to treat disease due to nematodes. The plants could be used as alternative anthelmintic to fight against Human and Bovine onchocerciasis.","PeriodicalId":9320,"journal":{"name":"British journal of pharmaceutical research","volume":"109 1","pages":"1-8"},"PeriodicalIF":0.0000,"publicationDate":"2017-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"British journal of pharmaceutical research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9734/BJPR/2017/33381","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
摘要
开发新型药物的策略之一是利用植物等天然资源进行治疗。植物提取物是一种具有协同作用的混合物,可以提高治疗效果,缩短治疗时间和减少耐药性。采用产自喀麦隆苏丹-几内亚和苏丹-萨赫勒地区的Cucurbita pepo ovifera var ovifera叶片和种子的乙醇提取物对牛寄生线虫ochengi盘尾丝虫进行了研究。用不同浓度的植物提取物在RPMI-1640中添加链霉素和庆大霉素孵育蠕虫。在37℃下监测24、48和72 h后的死亡率。以伊维菌素为阳性对照,以DMSO为阴性对照。植物提取物;生物工程学报,17(2):1-8,2017;文章no.BJPR。2个生态区的33381 2在72 h后对ochengi有一定的杀虫活性,LC50在20 ~ 1090 μg /mL之间。在苏丹-几内亚地区,叶提取物的抗丝活性最高(LC50为20 μg/mL),而在苏丹-萨赫勒地区,种子提取物的抗丝活性最高(LC50为17 μg/mL), 72 h后的抗丝活性与植物部位和生态区有关。此外,这种植物是无毒的。这些关于C. pepo ovifera var ovifera叶片和种子的乙醇提取物的结果证实了喀麦隆在传统医学中使用该植物治疗由线虫引起的疾病。该植物可作为防治人盘尾丝虫病和牛盘尾丝虫病的替代驱虫剂。
Antifilarial Activity of Cucurbita pepo ovifera var ovifera (Cucurbitaceae) on Onchocerca ochengi Adult Worms
One of the strategies for developing novel pharmaceutical drugs is to use natural sources such as plants for therapeutic treatment. Plant extracts are a cocktail of compounds which act synergically and can improve treatment effectiveness, reduce therapeutic duration and resistance. The ethanolic extracts of leaves and seeds of Cucurbita pepo ovifera var ovifera from Sudano-Guinean and Sudano-Sahelian zones of Cameroon were evaluated on the cattle parasite nematode Onchocerca ochengi. Worms were incubated with different concentrations of the plant extracts in RPMI-1640 supplemented with streptomycin and gentamicin. Mortality at 37°C was monitored after 24, 48 and 72 h. Ivermectin was used as positive control and DMSO as negative. Plant extracts Original Research Article Kalmobé et al.; BJPR, 17(2): 1-8, 2017; Article no.BJPR.33381 2 from the two ecological zones showed anthelminthic activities on O. ochengi after 72 h with LC50 varying from 20 to 1090 μg /mL. The highest antifilarial activity in Sudano-Guinean zone was obtained with leave extract of C. pepo ovifera (LC50 of 20 μg/mL), while highest antifilarial activity in Sudano-Sahelian zone was obtained with seed extracts of the plant with LC50 value of 17 μg/mL after 72 h. These results show that anthelmintic activity depends on the part of the plant and the ecological zones. Additionally, the plant is not toxic. These results on the ethanolic extracts of leaves and seeds of C. pepo ovifera var ovifera confirmed the use of this plant in traditional medicine in Cameroon to treat disease due to nematodes. The plants could be used as alternative anthelmintic to fight against Human and Bovine onchocerciasis.