喜树碱:溶解度、体外药物释放、对人红细胞和精子低温保存的影响。

IF 1 4区 生物学 Q3 BIOLOGY
S. Fatmi, L. Taouzinet, M. Skiba, M. Iguer-ouada
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引用次数: 0

摘要

喜树碱(CPT)是一种抗癌药物,由于其高疏水性和低活性形式稳定性而未用于临床。CPT在低温保存方面也有潜在的应用。目的通过通口溶解度实验研究环糊精(CDs)和聚乙二醇(PEG)存在下CPT的溶解度变化。材料与方法采用共蒸发法将scpt包封在不同的环糊精和聚乙二醇中。利用傅里叶变换红外光谱(FT-IR)和x射线粉末衍射(XRPD)研究了CPT与CDs和PEG 6000的相互作用。然后,评估CPT配合物的体外药物释放。为了评估CPT复合物体系的潜在抗癌功效,我们利用紫外分光光度法对人红细胞进行了体外细胞毒性研究。利用CASA研究了CPT复合物对4℃低温保存过程中精子活力保护的影响。结果这些制剂的溶出谱表明,在不同的扩散后,CPT的溶出度有所提高。CPT溶解度和稳定性的提高是红细胞试验细胞毒性的原因。然而,CPT单独、封装、分散和化学修饰在冷冻保存过程中保护精子。结论CPT包封和分散在抗癌治疗中具有重要意义。我们还发现包裹或分散的CPT可以保护精子免受氧化损伤,提高人类精子膜的完整性。因此,CPT封装我们的分散最终可能有利于不孕症的治疗。Doi: 10.54680 / fr23210110712。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Camptothecin: solubility, in-vitro drug release, and effect on human red blood cells and sperm cold preservation.
BACKGROUND Camptothecin (CPT) is an anticancer drug, and is not employed in the clinic because of its high hydrophobicity and low active form stability. CPT may also have potential for use in cold preservation. OBJECTIVE To overcome these drawbacks, CPT solubility variations in the presence of cyclodextrins (CDs) and polyethylene glycol (PEG) were evaluated by Higuchi solubility experiments. MATERIALS AND METHODS CPT was encapsulated in different cyclodextrins and polyethylene glycol using a co-evaporation method. The CPT interactions with CDs and PEG 6000 were investigated by Fourier-transformed infrared spectroscopy (FT-IR), and X-ray powder diffraction (XRPD). Then, CPT complexes were evaluated for in-vitro drug release. To evaluate the potential anticancer efficacy of the CPT complexes system, in-vitro cytotoxicity studies on human red blood cells were carried out using UV assay. The impact of the CPT complex systems on sperm motility protection during cold preservation at 4 degree C was studied using CASA. RESULTS The dissolution profile of these preparations shows the improvement of the dissolution of the CPT following a fickien diffusion. The CPT solubility and stability improvement were the cause of the cytotoxicity on the red blood cells test. However, CPT alone, encapsulated, dispersed, and chemically modified protected spermatozoids during cold preservation. CONCLUSION We confirm the interest in CPT encapsulated and dispersed in anticancer treatments. We also found that CPT encapsulated or dispersed could protect sperm against oxidative damage and improve the membrane integrity of human sperm. Consequently, CPT encapsulated our dispersed could eventually be beneficial for infertility therapy. Doi: 10.54680/fr23210110712.
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来源期刊
Cryo letters
Cryo letters 生物-生理学
CiteScore
1.80
自引率
10.00%
发文量
50
审稿时长
1 months
期刊介绍: A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
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