积雪草次生代谢产物的植物化学分析及抗氧化活性的紫外可见光谱评价

Current Biotechnology Pub Date : 2020-11-16 DOI:10.2174/2211550109999200821155854

Shouvik Chakravarty, K. Sultana, Sumanta Das, Indrani Chandra

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引用次数: 0

摘要

积雪草(Centella asiatica, L.)是一种多年生草本植物，属于蜂科。它富含重要的次生代谢物，如萜类、黄酮类和皂苷。它被用作治疗皮肤感染、咳嗽和哮喘的传统药物。本研究旨在对其次生代谢产物进行定量和定性分析。采用薄层色谱法(TLC)对化合物进行保留系数(Rf)分离。folin Ciocalteu法测定乙醇叶提取物总酚含量为45.72±6.05 mg GAE/g。水提液中总黄酮含量最高，为44.62±2.45 mg QE/g。叶片水提液和茎水提液对dpph(2,2-二苯基-1-苦味酰肼)的清除活性最高(分别为45.66±1.29和45.20±3.0)。本研究揭示了积雪草的抗氧化作用。

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Phytochemical Analysis of Secondary Metabolites and Evaluation of the Antioxidant Activity of Centella asiatica (L.) by UV-Vis Spectroscopy

Centella asiatica (L.) is an herbaceous, frost-tender perennial plant belonging to the Apiaceae family. It is rich in important secondary metabolites such as terpenoids, flavonoids and saponins. It is used as a traditional medicine for skin infection, cough and asthma. The present study was intended at the quantitative and qualitative analysis of its secondary metabolites. Thin Layer Chromatography (TLC) was performed to separate the compounds according to their Rf (Retention factor) values. The total phenolic content of ethanol leaf extract was 45.72 ± 6.05 mg GAE/g determined by Folin Ciocalteu’s assay. The maximum content of total flavonoids was found in aqueous leaf extract, 44.62 ± 2.45 mg QE/g. Aqueous extract of leaf and stem showed the highest antioxidant activity with DPPH (2,2- diphenyl-1-picrylhydrazyl) scavenging assay (45.66 ± 1.29 & 45.20 ± 3.0, respectively). The present study revealed the antioxidant properties in Centella asiatica.

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Current Biotechnology

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