芫荽(Coriandrum sativum L.)种子生理电位测定的样本大小

É. F. Leão-Araújo, Anderson Rodrigo da Silva, Amanda R. P. dos Santos, Kaique Ferreira Alves, L. D. Ferreira, H. A. Santana, Wilhan Valasco dos Santos, Brunna Rithielly Rezende
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引用次数: 0

摘要

本研究的目的是确定最小重复数和每次重复的种子数,以进行香菜种子的发芽和种子活力测试。对两个种子批次的含水量、活力和活力进行了比较。每批使用了800颗种子。分析了发芽率、发芽率、发芽速度指数和平均发芽时间。通过采用替代技术进行重采样,利用重复次数(2 ~ 10次)和每次重复种子数量(20 ~ 80次)的不同组合开发了样本量方案。参考样本由50个种子的4个重复组成,因为它通常用于对该物种的研究。最小重复数和种子数的确定是基于将每种情况的变异指数(CV/n0.5)的95%自举置信区间与参考样本的置信区间进行比较。为了确定发芽头数、发芽率和发芽速度指数,将每次复制的种子数从50粒减少到30粒是合理的。建议每次复制40粒种子来量化平均发芽时间。然而,重复次数的减少会对发芽和活力试验的准确性产生负面影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Sample size for determination of the physiological potential of coriander (Coriandrum sativum L.) seeds
The objective of this work was to determine the minimum number of replications and seeds per replication to perform the germination and seed vigor tests with coriander seeds. Two seed lots were compared in terms of water content, vigor and viability. Eight hundred seeds per lot were used. Values of germination first count, germination, germination speed index and mean germination time were analyzed. Sample size scenarios were developed using different combinations of number of replications (from 2 to 10) and the number of seeds per replication (from 20 to 80) by means of a resampling with replacement technique. The reference sample consisted of four replications of 50 seeds, as it is commonly used in researches with this species. Determination of the minimum number of replications and seeds was based on comparing the 95% bootstrapped confidence intervals for the index of variation (CV/n0.5) of each scenario with the confidence interval of the reference sample. It is reasonable to reduce the number of seeds per replication from 50 to 30 in order determine germination first count, germination and germination speed index. Forty seeds per replication are recommended to quantify the mean germination time. However, reductions in the number of replications can affect negatively the accuracy of germination and vigor tests.
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