Investigation of Different Extraction Methods on Antioxidant and Antimicrobial Activities of Teucrium hyrcanicum L.

Introduction: Medicinal plants are valuable sources of different antioxidant and antimicrobial compounds. In this study, the effects of different extraction methods with aqueous and methanolic solvents were investigated on the antioxidant and antimicrobial activities of Teucrium hyrcanicum L. Material & Methods: In this basic experimental study, aqueous and methanolic extraction was carried out using three methods, including a magnetic stirrer, a Bain-Marie bath, and Shaker. The antioxidant effects were then determined using DPPH and RP methods along with the determination of total phenol and total flavonoid. The antibacterial effect of aqueous and methanolic extracts against Staphylococcus aureus , Bacillus cereus, and Escherichia coli was determined through the disk diffusion method. ( Ethic code: IR.ausmt.rec.1398.11.34) Findings: The evaluation of antioxidant properties showed that methanolic extract had a stronger effect than aqueous extract. Moreover, it was demonstrated that the magnetic stirrer method was superior to the other two extraction methods. The same results were obtained for total phenol and total flavonoid as well. Evaluation of antibacterial properties revealed that methanolic extract had antibacterial activity, while aqueous extract did not show antibacterial activity. Discussion & Conclusion: The results of this study showed that Teucrium hyrcanicum L. was more effectively extracted through the magnetic stirrer method than other extraction methods. Both aqueous and methanolic extracts had antioxidant properties, while only methanol extracts showed antibacterial properties. Therefore, this plant can have good therapeutic application in the treatment of diseases due to its antioxidant properties.