{"title":"细菌重组肌生长抑制素的制备诱导合成肌生长抑制素特异性自身抗体","authors":"E. Koloskova, V. Ezerskiy, O. Zhukova","doi":"10.33647/2074-5982-18-3-22-26","DOIUrl":null,"url":null,"abstract":"The myostatin protein, belonging to the family of growth factors, represents a potential target for therapeutic effects in muscular system pathologies. However, this protein is characterized by other beneficial properties. Polymorphisms of the myostatin gene associated with the restriction of its functional activity are useful as genetic markers of meat productivity in farm animals. Blocking the action of myostatin in productive animals can be achieved by inducing the synthesis of specific autoantibodies using recombinant myostatin, possessing sufficient immunogenicity against myostatin as an antigen. A genetic construct was created and an E. coli producer strain with a high level of expression of recombinant myostatin was obtained.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"85 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Preparation of Bacterial Recombinant Myostatin to Induce the Synthesis of Myostatin-Specific Autoantibodies\",\"authors\":\"E. Koloskova, V. Ezerskiy, O. Zhukova\",\"doi\":\"10.33647/2074-5982-18-3-22-26\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The myostatin protein, belonging to the family of growth factors, represents a potential target for therapeutic effects in muscular system pathologies. However, this protein is characterized by other beneficial properties. Polymorphisms of the myostatin gene associated with the restriction of its functional activity are useful as genetic markers of meat productivity in farm animals. Blocking the action of myostatin in productive animals can be achieved by inducing the synthesis of specific autoantibodies using recombinant myostatin, possessing sufficient immunogenicity against myostatin as an antigen. A genetic construct was created and an E. coli producer strain with a high level of expression of recombinant myostatin was obtained.\",\"PeriodicalId\":14837,\"journal\":{\"name\":\"Journal Biomed\",\"volume\":\"85 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-10-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal Biomed\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.33647/2074-5982-18-3-22-26\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal Biomed","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33647/2074-5982-18-3-22-26","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Preparation of Bacterial Recombinant Myostatin to Induce the Synthesis of Myostatin-Specific Autoantibodies
The myostatin protein, belonging to the family of growth factors, represents a potential target for therapeutic effects in muscular system pathologies. However, this protein is characterized by other beneficial properties. Polymorphisms of the myostatin gene associated with the restriction of its functional activity are useful as genetic markers of meat productivity in farm animals. Blocking the action of myostatin in productive animals can be achieved by inducing the synthesis of specific autoantibodies using recombinant myostatin, possessing sufficient immunogenicity against myostatin as an antigen. A genetic construct was created and an E. coli producer strain with a high level of expression of recombinant myostatin was obtained.