大鼠脑特定神经元表达的高尔基体亮氨酸重复复合体CLRP的cDNA克隆及mRNA表达。

Julio Pérez-Márquez, Begoña Reguillo, R. Paniagua
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引用次数: 7

摘要

我们报道了从大鼠脑中分离的一种新的cDNA的分子克隆。我们将推测的蛋白质命名为CLRP,即复杂亮氨酸重复蛋白。预测的CLRP氨基酸序列与半乳糖、n -乙酰氨基葡萄糖和唾液酸转运蛋白在氨基酸组成上具有同源性,与一个人类5号染色体BAC克隆序列的同源性为91%。在COS-7细胞中,GFP标记的CLRP cDNA在细胞器中表达,细胞器类似于细胞质的高尔基体。在Northern blot中,CLRP探针在脑、肾、肺、睾丸和前列腺中标记了一个2.4 kb的单条带。在大脑中,CLRP mRNA由有限的神经元表达,如皮层的锥体细胞、小脑的浦肯野细胞和脑干的运动神经元。在大脑中,CLRP mRNA在胚胎第15天表达;表达水平维持到出生后第10天,在成人中下降。结果表明,CLRP编码了高尔基体核苷糖蛋白家族的新成员。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cloning of the cDNA and mRNA expression of CLRP, a complex leucine repeat protein of the Golgi apparatus expressed by specific neurons of the rat brain.
We report the molecular cloning of one novel cDNA isolated from the rat brain. We have named the putative protein CLRP, for complex leucine-repeat protein. The predicted CLRP amino acid sequence shares homology in the amino acid composition with the Galactose, N-Acetylglucosamine, and Sialic acid transporters, and shows 91% identity with the sequence of one human chromosome 5 BAC clone. Expression of the CLRP cDNA tagged with GFP in COS-7 cells was found in cell organelles that resemble the Golgi apparatus of the cytoplasm. In Northern blot, the CLRP probe labels a single band of 2.4 kb in the brain, kidney, lung, testis, and prostate. In the brain, CLRP mRNA is expressed by limited sets of neurons, such as the pyramidal cells of the cortex, the Purkinje cells of the cerebellum, and the motoneurons of the brainstem. In the brain, the CLRP mRNA is expressed at embryonic day 15; levels of expression are maintained until postnatal day 10 and decrease in adults. The results suggest that CLRP codes a novel member of the nucleotide-sugar family of proteins of the Golgi apparatus.
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