牙源性角化囊肿的上皮-间充质标志物及其与临床的相关性

J. Vendruscolo, Mariana de SOUZA LESSA, Sergio OSSAMU IOSHII, J. Schussel, L. Sassi
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引用次数: 0

摘要

摘要目的:牙源性角化囊肿具有高复发率和侵袭性临床行为。上皮-间充质转化是上皮细胞失去其上皮特性并获得间充质细胞典型特性的过程。研究表明,牙源性角化囊肿具有肿瘤标志物的表达,但其发生机制和分子组成尚不明确,使得治疗选择仍然有限。本研究的目的是评估这些病变中上皮-间质过渡标记蛋白的表达,并将这些蛋白的表达与每个病例的临床方面联系起来。方法:对2016 - 2019年在巴西库里蒂巴市Erasto Gaertner医院口腔颌面外科治疗的牙源性角化囊肿患者进行免疫组化分析,定性分析上皮-间质转化标志物(Vimentin、β -catenin、E-cadherin)的表达情况。结果:纳入18例患者,平均年龄43岁,男性居多。下颌骨比上颌骨受影响更大。没有观察到囊肿的临床特征与上皮-间充质转化蛋白的免疫组织化学特征之间的关联。结论:E-cadherin呈阳性,而vimentin呈阴性,表明其功能得以保留。e -钙粘蛋白功能丧失与预后不良相关。鉴定上皮-间质转化过程作为牙源性囊肿和肿瘤的预后标志物可能是确定治疗的重要工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Epithelial-mesenchymal markers and their correlation with clinical aspects in odontogenic keratocysts
ABSTRACT Objective: Odontogenic keratocysts have a high recurrence rate and aggressive clinical behavior. The event called epithelial-mesenchymal transition is a process in which the epithelial cell loses its epithelial characteristics and acquires properties typical of mesenchymal cells. Studies have already demonstrated that odontogenic keratocysts has expression of tumor markers, but the lack of clarification about its development mechanism and molecular composition makes the therapeutic options remain limited. The aim of this study is to evaluate the expression of epithelial-mesenchymal transition marker proteins in these lesions, correlating the expression of these proteins with clinical aspects of each case. Methods: Patients with odontogenic keratocysts diagnoses, treated by the Department of Oral and Maxillofacial Surgery of the Erasto Gaertner Hospital, Curitiba, Brazil in the period between 2016 and 2019 were evaluated by immunohistochemical analysis, to assess the expression of epithelial-mesenchymal transition markers (Vimentin, beta-catenin and E-cadherin) by qualitative analysis. Results: Eighteen patients were included, with a mean age of 43 years, and most of them were male. The mandible was more affected than the maxilla. No association between the clinical characteristics of the cysts and the immunohistochemical profile for epithelial-mesenchymal transition proteins was observed. Conclusion: The positivity of E-cadherin and negativity of vimentin demonstrates that its function is preserved. Loss of function of E-cadherin is associated with worse prognosis. The identification of the epithelial-mesenchymal transition process as a prognostic marker for odontogenic cysts and tumors could be an important tool for defining treatment.
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