Phytochemical, elemental, physico-chemical, HPTLC and anticancer investigations of Ceropegia spiralis Wight. Tuber extracts

Phytochemical constituents are responsible for medicinal activity of plant species. Hence in the present study Qualitative and quantitative phytochemical screening, Physicochemical, elemental analysis and anticancer activity of Ceropegia spiralis tuber extracts were carried out. Qualitative and quantitative phytochemical analysis of tuber aqueous extract confirm the presence of various secondary metabolites like saponins, triterpenoids, steroids, tannins, alkaloids, flavonoids and phenols. The results suggest that the phytochemical properties for curing various ailments and possess potential anticancer, antimicrobial and antioxidant activities leads to the isolation of new and novel compounds. Physicochemical studies reveals that the dry matter 96.23%; followed by Water soluble extractive 14.28 %. Elemental analysis reveals Nitrogen 3.4 % followed by Zinc 218.9%. Along with this, macro and micro elements which are essential for maintaining the animal body were also determined quantitatively. Anticancer activity of C. spiralis exhibited potential towards MDAMB-231 (human breast cancer) cell lines, shows 78.30% cell death with cell viability 21.70% at 100 µg/ml. The presence of various bioactive compounds confirms the application of C. spiralis against many ailments by the traditional practitioners.