橘皮诱导普鲁兰小孢子菌细胞壁降解酶的动力学和热力学研究:在番茄红素提取中的应用

A. Ademakinwa, F. Agboola
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引用次数: 17

摘要

摘要:本文研究了柑橘皮诱导褐毛小孢子虫NAC8产生纤维素酶和果胶酶等细胞壁降解酶,探讨了这些酶在番茄皮、废、膏中番茄红素提取中的潜在应用。然后通过海藻酸盐珠包埋固定化CWDE提取番茄红素,并研究了游离和固定化CWDE的动力学/热力学性质。在pH为6.0、温度为50℃、果皮浓度为2.0% (w/v)的条件下,CWDE的最佳产量最高。得到的动力学和热力学参数如和的数值表明,游离和固定化的纤维素酶和果胶酶在40 ~ 50℃之间都是耐热的。固定化CWDE提取的番茄红素最高可达80±2.4 mg/kg、42±1.3 mg/kg和60±1.2 mg/kg。第5次循环后,包埋CWDE对番茄红素的提取率分别为58±4.2、51±1.2和57±4.2%。利用柑桔皮诱导葡聚糖CWDE为从番茄SWP中提取番茄红素提供了一种独特而廉价的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Kinetic and thermodynamic investigations of cell-wall degrading enzymes produced by Aureobasidium pullulans via induction with orange peels: application in lycopene extraction
Abstract The production of cell-wall degrading enzymes (CWDE) such as cellulase and pectinase by Aureobasidium pullulans NAC8 through induction using orange peels was investigated for the potential application of these enzymes in the extraction of lycopene from tomato skin, waste, and paste (SWP). The CWDE was then immobilized via entrapment in alginate beads for lycopene extraction and the kinetic/thermodynamic properties of the free and immobilized CWDE investigated. The optimum production of CWDE occurred at pH, temperature, and orange peel concentration of 6.0, 50 °C, and 2.0% (w/v), respectively. The values obtained for some kinetic and thermodynamic parameters such as and indicate that both free and immobilized cellulase and pectinase were thermostable between 40 and 50 °C. Maximum lycopene extracted from the tomato SWP was 80 ± 2.4 mg/kg, 42 ± 1.3 mg/kg and 60 ± 1.2 mg/kg, respectively, using the immobilized CWDE. The entrapped CWDE was able to extract lycopene with yields of 58 ± 4.2, 51 ± 1.2 and 57 ± 4.2% for tomato SWP respectively after the fifth cycle. Using orange peels for the induction of CWDE by A. pullulans offers a unique and cheaper approach to obtaining thermostable multi-enzyme complexes employable for easy lycopene extraction from tomato SWP.
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