天然类胡萝卜素结构解析与分析的实验技术

T. Maoka
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引用次数: 16

摘要

自1930年Kuhn和Karrer首次阐明β-胡萝卜素的结构以来,到2004年,大约有750种天然存在的类胡萝卜素被报道。核磁共振(NMR)、质谱(MS)、高效液相色谱(HPLC)等分析仪器的改进,使自然界中许多微量类胡萝卜素的结构解析成为可能。每年都有新的结构类胡萝卜素在自然界中被报道。本文综述了天然类胡萝卜素结构分析的实验技术,包括提取、分离、化学衍生化方法以及HPLC、UVVIS、MS、NMR、CD光谱分析等。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Experimental Techniques for Structural Elucidation and Analysis of Natural Carotenoids
Since the first structural elucidation of β-carotene by Kuhn and Karrer in 1930, about 750 naturally occurring carotenoids had been reported till 2004. Improvements of analytical instruments such as NMR, MS, HPLC, etc., have made it possible to per form the structural elucidation of ver y minor carotenoids in nature. Annually, new structural carotenoids are being reported in nature. The present paper provides a review of experimental techniques for structural elucidation and analysis of natural carotenoids including extraction, isolation, chemical derivatization methods and HPLC, UVVIS, MS, NMR, CD spectrometry.
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