Initial Studies on Isolation and Characterization of Antioxidant Compounds from Immature Berries of Ampelopsis grandulosa

This initial study is aimed to isolate and characterize the antioxidant compounds from immature berries of Ampelopsis grandulosa (Vitaceae). In Japan, A. grandulosa is utilized for folk medicine because some local people in Japan believe that it has beneficial effects to human’ health. However, the studies of bioactive compounds in particular antioxidant activity has not been reported for A. grandulosa. The antioxidant activities were monitored using 2,2-dyphenyl-1-perylhydrazyl (DPPH) method. The chemical structures of active compounds were determined using spectroscopic techniques such as GC-MS, H NMR, C/DEPT NMR, COSY, HMQC, and HMBC. A mixture of fatty acids containing palmitic acid, stealic acid, oleic acid, palmitoleic acid, trans-palmitoleic acid, and linoleic acid exhibit the radical scavenging activity. In addition, (+)-catechin and ethyl gallate have been also isolated as powerful antioxidant. Keywords—Antioxidant compounds, DPPH, radical scavenging, Ampelopsis grandulosa.