A. Sysa, M. Labai, E. Kvasyuk, Uliana Ivuts, Maksim Khanchevskii
{"title":"阿拉伯糖脲基胞嘧啶-5′-单磷酸及其莫西平盐对外周血淋巴细胞亚群活力和功能状态的影响","authors":"A. Sysa, M. Labai, E. Kvasyuk, Uliana Ivuts, Maksim Khanchevskii","doi":"10.33945/sami/pcbr.2019.4.3","DOIUrl":null,"url":null,"abstract":"The influence of the modified nucleotide arabinofuranosylcytosine-5`-monophosphate in the form of the free acid (Ara-CMP) and its salts with emoxipin (Ara-CMP+Em) on the viability of peripheral blood mononuclear cells, the lymphocyte proliferation of mitogen-stimulated cells was studied. It was shown that emoxipin - the compound with strong antioxidant properties - does not lead to inhibition of the main function of the antimetabolite Ara-CMP, namely, cell growth arrest (p = 0.96) and lysis of target cells (p = 0.97). Under the conditions of nonspecific stimulation of lymphocytes, Ara-CMP at high concentrations (10-4 M) increased in both IFNγ-secreting T-lymphocytes (five times) and IFNγ-secreting other lymphocytes (three times). Emoxipin led to almost complete leveling of the detected effect.","PeriodicalId":20668,"journal":{"name":"Progress in Chemical and Biochemical Research","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Influence of arabinofuranosylcytosine-5`-monophosphate and its emoxipin salt on viability and functional status of peripheral blood lymphocytes subpopulations\",\"authors\":\"A. Sysa, M. Labai, E. Kvasyuk, Uliana Ivuts, Maksim Khanchevskii\",\"doi\":\"10.33945/sami/pcbr.2019.4.3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The influence of the modified nucleotide arabinofuranosylcytosine-5`-monophosphate in the form of the free acid (Ara-CMP) and its salts with emoxipin (Ara-CMP+Em) on the viability of peripheral blood mononuclear cells, the lymphocyte proliferation of mitogen-stimulated cells was studied. It was shown that emoxipin - the compound with strong antioxidant properties - does not lead to inhibition of the main function of the antimetabolite Ara-CMP, namely, cell growth arrest (p = 0.96) and lysis of target cells (p = 0.97). Under the conditions of nonspecific stimulation of lymphocytes, Ara-CMP at high concentrations (10-4 M) increased in both IFNγ-secreting T-lymphocytes (five times) and IFNγ-secreting other lymphocytes (three times). Emoxipin led to almost complete leveling of the detected effect.\",\"PeriodicalId\":20668,\"journal\":{\"name\":\"Progress in Chemical and Biochemical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Progress in Chemical and Biochemical Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.33945/sami/pcbr.2019.4.3\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Progress in Chemical and Biochemical Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33945/sami/pcbr.2019.4.3","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Influence of arabinofuranosylcytosine-5`-monophosphate and its emoxipin salt on viability and functional status of peripheral blood lymphocytes subpopulations
The influence of the modified nucleotide arabinofuranosylcytosine-5`-monophosphate in the form of the free acid (Ara-CMP) and its salts with emoxipin (Ara-CMP+Em) on the viability of peripheral blood mononuclear cells, the lymphocyte proliferation of mitogen-stimulated cells was studied. It was shown that emoxipin - the compound with strong antioxidant properties - does not lead to inhibition of the main function of the antimetabolite Ara-CMP, namely, cell growth arrest (p = 0.96) and lysis of target cells (p = 0.97). Under the conditions of nonspecific stimulation of lymphocytes, Ara-CMP at high concentrations (10-4 M) increased in both IFNγ-secreting T-lymphocytes (five times) and IFNγ-secreting other lymphocytes (three times). Emoxipin led to almost complete leveling of the detected effect.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
