T. Puangkrit, T. Narumi-Kawasaki, T. Takamura, S. Fukai
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引用次数: 5
摘要
严重的问题。为了确定高温对色素沉着的影响,将花序发育分为五个阶段。植株在花序发育的不同阶段分别暴露于20℃和30℃。HPLC分析表明,红菊(cv;分别为花青素3- 0 -(6≤- o -单甘油酯- b -葡萄糖苷)和花青素3- 0 -(3≤,6≤- o -二丙二醇基- b -葡萄糖苷)。两种花青素在20℃处理下的含量明显高于30℃处理。从芽裂期到立交期,即使在立交期至1周龄期间,在20℃下处理,30℃下的花序色素沉着也没有增强。另一方面,从芽裂期到立交期,即使将花序保持在20℃,当植株垂直期至1周龄暴露在30℃时,色素含量也急剧下降。结果表明,花瓣伸展至垂直阶段对温度最敏感,对色素沉着最重要。花青素生物合成相关基因(cplchs1、cplchs2、cplchi、cplf3h2、cplc3’h、cpldfr1、cpldfr2和cplplans)在30℃下的表达比在20℃下降低。
Inflorescence Developmental Stage-Specific High Temperature Effect on Petal Pigmentation in Chrysanthemum
serious problem. To determine the effect of high temperature on the pigmentation, inflorescence development was divided into five stages. Plants were exposed to both 20 and 30 ℃ during various developmental stages of inflorescence. HPLC analysis showed the main anthocyanins of pink flower chrysanthemum (cv. Pelican) were cyanidin 3-O-(6 ≤ -O-monomalonyl- b -glucopyranoside) and cyanidin 3-O-(3 ≤ ,6 ≤ -O-dimalonyl- b -glucopyranoside). The content of the two anthocyanins at 20 ℃ was much higher than that at 30 ℃ . In the inflorescence exposed to 30 ℃ during bud break to vertical stage, pigmentation was not enhanced, even though the plants were subjected to 20 ℃ from the vertical stage to 1-week-old. On the other hand, when the plants were exposed to 30 ℃ during vertical stage to 1-week-old, pigment content decreased drastically, even though the inflorescence was kept at 20 ℃ from the bud break to vertical stage. The results indicate that the petal extension to vertical stage is the most temperature sensitive and important for pigmentation. Expression of the anthocyanin biosynthesis-related genes ( CmplCHS1 , CmplCHS2 , CmplCHI , CmplF3H2 , CmplC3’H , CmplDFR1 , CmplDFR2 , and CmplANS ) was depressed at 30 ℃ compared with those at 20 ℃ .