波波附藤内生真菌的分离鉴定及药理活性研究。

N. Chowdhury, Farhana Farjana, M. Sohrab
{"title":"波波附藤内生真菌的分离鉴定及药理活性研究。","authors":"N. Chowdhury, Farhana Farjana, M. Sohrab","doi":"10.4236/pp.2020.1112028","DOIUrl":null,"url":null,"abstract":"The objective of this study \nis to isolate, identify and investigate the \npharmacological activities of the endophytic fungi from an aquatic plant Aponogeton undulatus Roxb. (A. \nundulatus). Endophytic fungi were isolated and identified based \non morphological characters. The \nmolecular identification of the fungal isolates was performed using by \nanalyzing the DNA sequence based on mega BLAST program. Spectrums of \nantibacterial and antifungal activities were \nstudied by Agar diffusion methods. Extracts of the endophytic fungal \nstrains isolated from the plant A. undulatus were screened for probable \ncytotoxic activities using brine shrimp lethality \nbioassay. Six endophytic fungi, namely AULE-1, AULE-2, AULE-3, AURE-1, AURE-3 and AURE-4 were isolated and purified from the leaves and roots of the plant such as strains AULE-1 as Trichooderma sp., \nAULE-2 as Carvularia sp. AULE-3 as Penicillium sp. strains AURE-1 \nand AURE-4 as Fusarium sp. and AURE-3 as Mucor sp. Strain AURE-4 was further identified as Fusarium solani. At 300 μg/disc concentration, six ethyl acetate extracts of endophytic \nfungi of the plant A. undulates showed \nmoderate to significant activities against \nmost of the test bacteria and pathogenic fungi. The strain AURE-4 \nexhibited strong cytotoxic activities (10.18 μg/mL) compared to the standard \nantitumor agent vincristine sulphate and this \npositive result suggests that fungal extracts may contain antitumor or \npesticidal compounds. This is the first study to report the isolation, \nidentification, antimicrobial and cytotoxic properties of endophytic fungi of Aponogeton undulatus Roxb. in \nBangladesh.","PeriodicalId":19875,"journal":{"name":"Pharmacology & Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Isolation, Identification and Pharmacological Activities of Endophytic Fungi from Aponogeton undulatus Roxb.\",\"authors\":\"N. Chowdhury, Farhana Farjana, M. Sohrab\",\"doi\":\"10.4236/pp.2020.1112028\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The objective of this study \\nis to isolate, identify and investigate the \\npharmacological activities of the endophytic fungi from an aquatic plant Aponogeton undulatus Roxb. (A. \\nundulatus). Endophytic fungi were isolated and identified based \\non morphological characters. The \\nmolecular identification of the fungal isolates was performed using by \\nanalyzing the DNA sequence based on mega BLAST program. Spectrums of \\nantibacterial and antifungal activities were \\nstudied by Agar diffusion methods. Extracts of the endophytic fungal \\nstrains isolated from the plant A. undulatus were screened for probable \\ncytotoxic activities using brine shrimp lethality \\nbioassay. Six endophytic fungi, namely AULE-1, AULE-2, AULE-3, AURE-1, AURE-3 and AURE-4 were isolated and purified from the leaves and roots of the plant such as strains AULE-1 as Trichooderma sp., \\nAULE-2 as Carvularia sp. AULE-3 as Penicillium sp. strains AURE-1 \\nand AURE-4 as Fusarium sp. and AURE-3 as Mucor sp. Strain AURE-4 was further identified as Fusarium solani. At 300 μg/disc concentration, six ethyl acetate extracts of endophytic \\nfungi of the plant A. undulates showed \\nmoderate to significant activities against \\nmost of the test bacteria and pathogenic fungi. The strain AURE-4 \\nexhibited strong cytotoxic activities (10.18 μg/mL) compared to the standard \\nantitumor agent vincristine sulphate and this \\npositive result suggests that fungal extracts may contain antitumor or \\npesticidal compounds. This is the first study to report the isolation, \\nidentification, antimicrobial and cytotoxic properties of endophytic fungi of Aponogeton undulatus Roxb. in \\nBangladesh.\",\"PeriodicalId\":19875,\"journal\":{\"name\":\"Pharmacology & Pharmacy\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-12-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmacology & Pharmacy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4236/pp.2020.1112028\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacology & Pharmacy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4236/pp.2020.1112028","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2

摘要

本研究的目的是分离、鉴定和研究水生植物波波附藤内生真菌的药理活性。(答:波状云)。根据形态特征对内生真菌进行了分离鉴定。利用mega BLAST程序对分离菌株的DNA序列进行分析,对分离菌株进行分子鉴定。用琼脂扩散法研究了其抑菌和抗真菌活性。采用盐水对虾致死性生物测定法对从波状芽孢杆菌中分离得到的内生真菌提取物进行了细胞毒活性筛选。从该植物的叶片和根中分离纯化了6种内生真菌,分别为AULE-1、AULE-2、AULE-3、AULE-1、AURE-3和AURE-4,其中菌株AULE-1为Trichooderma sp.,菌株AULE-2为Carvularia sp.,菌株AULE-3为Penicillium sp.,菌株AULE-1和aule -4为Fusarium sp.,菌株AURE-4为Mucor sp.,进一步鉴定菌株AURE-4为枯萎菌。在300 μg/盘浓度下,6种乙酸乙酯提取物对大部分试验菌和病原菌均有抑制作用。与标准抗肿瘤剂硫酸长春新碱相比,菌株AURE-4表现出较强的细胞毒活性(10.18 μg/mL),表明真菌提取物可能含有抗肿瘤或杀虫化合物。本文首次报道了波波附根内生真菌的分离、鉴定、抑菌和细胞毒特性。在孟加拉国。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation, Identification and Pharmacological Activities of Endophytic Fungi from Aponogeton undulatus Roxb.
The objective of this study is to isolate, identify and investigate the pharmacological activities of the endophytic fungi from an aquatic plant Aponogeton undulatus Roxb. (A. undulatus). Endophytic fungi were isolated and identified based on morphological characters. The molecular identification of the fungal isolates was performed using by analyzing the DNA sequence based on mega BLAST program. Spectrums of antibacterial and antifungal activities were studied by Agar diffusion methods. Extracts of the endophytic fungal strains isolated from the plant A. undulatus were screened for probable cytotoxic activities using brine shrimp lethality bioassay. Six endophytic fungi, namely AULE-1, AULE-2, AULE-3, AURE-1, AURE-3 and AURE-4 were isolated and purified from the leaves and roots of the plant such as strains AULE-1 as Trichooderma sp., AULE-2 as Carvularia sp. AULE-3 as Penicillium sp. strains AURE-1 and AURE-4 as Fusarium sp. and AURE-3 as Mucor sp. Strain AURE-4 was further identified as Fusarium solani. At 300 μg/disc concentration, six ethyl acetate extracts of endophytic fungi of the plant A. undulates showed moderate to significant activities against most of the test bacteria and pathogenic fungi. The strain AURE-4 exhibited strong cytotoxic activities (10.18 μg/mL) compared to the standard antitumor agent vincristine sulphate and this positive result suggests that fungal extracts may contain antitumor or pesticidal compounds. This is the first study to report the isolation, identification, antimicrobial and cytotoxic properties of endophytic fungi of Aponogeton undulatus Roxb. in Bangladesh.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信