不同溶剂提取物主要生物活性成分及抗氧化活性的比较

F. Toul, Amina Djendar
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引用次数: 0

摘要

本研究考察了五种极性递增的溶剂对芒草提取物的化学成分和抗氧化活性的影响。反相高效液相色谱分析鉴定出12种酚类化合物,包括8种酚酸(咖啡酸、鞣花酸、阿魏酸、没食子酸、迷迭香酸、辛酸、丁香酸和香草酸)和4种黄酮类化合物(儿茶素、橙皮素、木犀草素和槲皮素)。使用两种不同的测试系统来评估抗氧化活性:DPPH和β-胡萝卜素/亚油酸测定。甲醇提取物对DPPH的清除能力最强,对β-胡萝卜素漂白的抑制作用较低;氯仿提取物对β-胡萝卜素漂白的清除能力最强,清除能力较弱。甲醇提取物中总酚含量最高(26.57±0.15 mg GAE/g E),氯仿提取物中总黄酮含量最高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison of Major Bioactive Components of Various Solvent Extracts from Algerian Bunium incrassatum Umbellules and Antioxidant Activity
The present study was designed to examine the chemical composition and the antioxidant activity of Bunium incrassatum extracts prepared in five different solvents with increasing polarities. RP-HPLC analysis resulted in the identification of 12 phenolic compounds, including 8 phenolic acids (caffeic acid, ellagic acid, ferulic acid, gallic acid, rosmarinic acid, sinapic acid, syringic acid, and vanillic acid) and 4 flavonoids (catechin, hesperetin, luteolin, and quercetin). Two different test systems were used to assess the antioxidant activity: DPPH and β-carotene/linoleic acid assays. Methanol extract exhibited the highest DPPH scavenging potential and even low inhibitory effect against β-carotene bleaching, whereas chloroform extract showed the highest effect against β-carotene bleaching and a weak scavenging potential. As expected, methanol extract recorded the highest content of total phenolics (26.57±0.15 mg GAE/g E), while chloroform extract has been found to be the richest in flavonoids.
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