{"title":"一株口服痤疮丙酸杆菌的磷脂酶C纯化及部分鉴定","authors":"Takeshi Nakamura , Hiroo Taniguchi , Kazunori Takeuchi , Setsuo Fujimura , Gerhard Pulverer","doi":"10.1016/S0174-3031(84)80039-6","DOIUrl":null,"url":null,"abstract":"<div><p>Phospholipase C of the oral <em>Propionibacterium acnes</em> strain D 7 was purified from culture supernatants and partially characterized. The molecular weight was found to be 32.000 and the optimum pH was situated between 7.0 to 8.0. This nonhemolytic enzyme hydrolyzed relative intensively acidic glycerophospholipids and produced 1,2-diglyceride from phosphatidyl choline.</p></div>","PeriodicalId":79282,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...","volume":"257 1","pages":"Pages 20-26"},"PeriodicalIF":0.0000,"publicationDate":"1984-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0174-3031(84)80039-6","citationCount":"0","resultStr":"{\"title\":\"Phospholipase C of an Oral Strain of Propionibacterium acnes Purification and Partial Characterization\",\"authors\":\"Takeshi Nakamura , Hiroo Taniguchi , Kazunori Takeuchi , Setsuo Fujimura , Gerhard Pulverer\",\"doi\":\"10.1016/S0174-3031(84)80039-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Phospholipase C of the oral <em>Propionibacterium acnes</em> strain D 7 was purified from culture supernatants and partially characterized. The molecular weight was found to be 32.000 and the optimum pH was situated between 7.0 to 8.0. This nonhemolytic enzyme hydrolyzed relative intensively acidic glycerophospholipids and produced 1,2-diglyceride from phosphatidyl choline.</p></div>\",\"PeriodicalId\":79282,\"journal\":{\"name\":\"Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...\",\"volume\":\"257 1\",\"pages\":\"Pages 20-26\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1984-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0174-3031(84)80039-6\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0174303184800396\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious...","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0174303184800396","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Phospholipase C of an Oral Strain of Propionibacterium acnes Purification and Partial Characterization
Phospholipase C of the oral Propionibacterium acnes strain D 7 was purified from culture supernatants and partially characterized. The molecular weight was found to be 32.000 and the optimum pH was situated between 7.0 to 8.0. This nonhemolytic enzyme hydrolyzed relative intensively acidic glycerophospholipids and produced 1,2-diglyceride from phosphatidyl choline.
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