Investigating of dietary nitrate and nitrite interaction and vitamin C and urinary and serum nitrate and nitrite levels: Tehran Lipid and Glucose Study

Background and Aim: studies show that antioxidants prevent conversion of NO3/NO2 to nitrosamines compounds. tha aim of this study was to determine the potential effect of interaction between dietary intakes of vitamin C and NO3/NO2 on urinary and serum levels of NO3/NO2. Materials and Methods: This cross-sectional study included 251 adults aged >19 years was performed in the phase 6 of the Tehran Lipid and Glucose Study in 2015. Food intake was assessed using a semi-quantitative food frequency questionnaire. The average daily intake of nitrate and nitrite and vitamin C was estimated. Serum and urine levels of nitrate and nitrite were measured using the Grease method. In order to determine the effect of vitamin C on the interaction of nitrate and nitrite in urine and serum, linear regression test was used to modify the effects of confounders. Results: There was a significant correlation between intake of nitrate and its urine level, above the median level of vitamin C intake (B = 0/004, P-value = 0/004). Also, this correlation remained significant after adjusting for age, sex, body mass index, calorie intake (B = 0.03, P-value = 0/001). The effect of vitamin C interactions on nitrate intake and urine level in the adjusted model for nitrate and nitrite was also found to be significant (B = 0/003 P-value = 0/001). Conclusion: Increased intake of vitamin C significantly increases urinary excretion of nitrate and it is effective in the interaction between dietary intakes and urinary excretion of nitrate. Keyword: nitrate, nitrite, vitamin C Received: Dec 4, 2018 Accepted: Dec 20, 2020 Copyright © 2018 the Author (s). Published by Kurdistan University of Medical Sciences. This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial License 4.0 (CCBYNC), where it is permissible to download, share, remix, transform, and buildup the work provided it is properly cited. The work cannot be used commercially without permission from the journal How to cite the article: Zahra Bahadoran , Zahra Saeedirad , Maryam Mousavi , Parvin Mirmiran , fereidoun Azizi. Investigating of dietary nitrate and nitrite interaction and vitamin C and urinary and serum nitrate and nitrite levels: Tehran Lipid and Glucose Study. ُ ُSJKU 2021;26(6):57-68.