{"title":"蛋白质纯化乙腈-水体系的液-液平衡","authors":"David N. Oence, Tingyue Gu","doi":"10.1016/S0956-9618(96)00161-0","DOIUrl":null,"url":null,"abstract":"<div><p>Liquid-Liquid Equilibria (LLE) were measured at atmospheric pressure for the binary acetonitrile-water system at temperatures ranging from −1.3 to −18.6°C. Data points were taken using two methods, the cloud point method and the analysis of phase compositions using gas chromatography. Both methods show excellent agreement in their representation of the phase envelope. Temperature dependent NRTL (non-random two liquid) parameters were calculated from the binary data. The parameters calculated were <em>τ</em><sub>12</sub> = 355.7−31.49<sub><em>T</em></sub>(°C) and <em>τ</em><sub>21</sub> = 967.2−26.59<em>T</em>(°C) with the a value held constant 0.3.</p></div>","PeriodicalId":101160,"journal":{"name":"Separations Technology","volume":"6 4","pages":"Pages 261-264"},"PeriodicalIF":0.0000,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0956-9618(96)00161-0","citationCount":"25","resultStr":"{\"title\":\"Liquid-liquid equilibrium of the acetonitrile-water system for protein purification\",\"authors\":\"David N. Oence, Tingyue Gu\",\"doi\":\"10.1016/S0956-9618(96)00161-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Liquid-Liquid Equilibria (LLE) were measured at atmospheric pressure for the binary acetonitrile-water system at temperatures ranging from −1.3 to −18.6°C. Data points were taken using two methods, the cloud point method and the analysis of phase compositions using gas chromatography. Both methods show excellent agreement in their representation of the phase envelope. Temperature dependent NRTL (non-random two liquid) parameters were calculated from the binary data. The parameters calculated were <em>τ</em><sub>12</sub> = 355.7−31.49<sub><em>T</em></sub>(°C) and <em>τ</em><sub>21</sub> = 967.2−26.59<em>T</em>(°C) with the a value held constant 0.3.</p></div>\",\"PeriodicalId\":101160,\"journal\":{\"name\":\"Separations Technology\",\"volume\":\"6 4\",\"pages\":\"Pages 261-264\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1996-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0956-9618(96)00161-0\",\"citationCount\":\"25\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Separations Technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0956961896001610\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Separations Technology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0956961896001610","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Liquid-liquid equilibrium of the acetonitrile-water system for protein purification
Liquid-Liquid Equilibria (LLE) were measured at atmospheric pressure for the binary acetonitrile-water system at temperatures ranging from −1.3 to −18.6°C. Data points were taken using two methods, the cloud point method and the analysis of phase compositions using gas chromatography. Both methods show excellent agreement in their representation of the phase envelope. Temperature dependent NRTL (non-random two liquid) parameters were calculated from the binary data. The parameters calculated were τ12 = 355.7−31.49T(°C) and τ21 = 967.2−26.59T(°C) with the a value held constant 0.3.
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